单个前列腺细胞g -肌动蛋白含量异常作为前列腺癌的生物标志物。

Cancer detection and prevention Pub Date : 2000-01-01
G P Hemstreet, R B Bonner, R E Hurst, D Bell, B L Bane
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引用次数: 0

摘要

本文研究了根治性前列腺切除术后27例切除前列腺,经细针穿刺(FNA)获得的前列腺细胞中改变的G-actin的分布。FNA用于获取单细胞用于图像分析,在任何结节区域和对侧叶的大致正常区域取样。定量荧光图像分析测定单个细胞中g -肌动蛋白的含量。异常的g -肌动蛋白,一种代表细胞转化过程中细胞骨架重排的前体细胞骨架蛋白,在27例显性结节标本中有22例与腺癌有关,但在20例大体正常标本中只有3例(P
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Abnormal G-actin content in single prostate cells as a biomarker of prostate cancer.

The distribution of altered G-actin was investigated in prostatic cells obtained by fine needle aspiration (FNA) from 27 excised prostate glands obtained during radical prostatectomy. FNA, which was used to obtain single cells for image analysis, sampled in the region of any nodules and in grossly normal areas of the contralateral lobes. Quantitative fluorescence-image analysis was used to assay the amount of G-actin in individual cells. Abnormal G-actin, a precursor cytoskeletal protein representing cytoskeletal rearrangements accompanying cellular transformation, was associated with the presence of adenocarcinoma in 22 of 27 specimens from the dominant nodule, but only 3 of 20 in the grossly normal specimens (P<.0001). The mean G-actin content of all samples from the dominant nodule was 113.2+/-6.87 and 69.57+/-4.47 from the grossly normal area, the difference being significant at P<.0001. Altered G-actin was not associated with Gleason score (P = .95), grade (P = .26), stage (P = .058), or tumor volume (P = .32), thereby indicating it is a general marker for prostate adenocarcinoma.

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