M.P. Gallegos-Arreola , L. Arnaud-López , L.E. Figuera , T.S.Beltrán Jaramillo , H. Rangel-Villalobos , Sunji Thomatsu , G.M. Zúñiga-González
{"title":"粘多糖病I型:墨西哥人群患者与健康人群Eco47III-NspI单倍型位点频率的比较研究","authors":"M.P. Gallegos-Arreola , L. Arnaud-López , L.E. Figuera , T.S.Beltrán Jaramillo , H. Rangel-Villalobos , Sunji Thomatsu , G.M. Zúñiga-González","doi":"10.1016/S0003-3995(03)00010-8","DOIUrl":null,"url":null,"abstract":"<div><p><strong><em>Background. –</em></strong> Mucopolysaccharidosis I (MPS-I) is an autosomal recessive disorder, which is caused by mutations in the IDUA gene. It induces the deficiency of glycosidase α-L-duronidase. The enzyme that is required for the degradation of heparan and dermatan sulfate. This disorder expresses a wide range of clinical symptoms (severe mental retardation, skeletal deformations, hepatosplenomegaly, corneal clouding and mild visceral organ involvement). In the present paper, we report the frequencies of haplotypes of the <em>Eco</em>47III–<em>Nsp</em>I sites, in the IDUA gene, in Mexican healthy and in MPS-I individuals.</p><p><strong><em>Methods. –</em></strong><em>Eco</em>47III and <em>Nsp</em>I intragenic polymorphisms in IDUA gene were studied in 262 (524 chromosomes) Mexican healthy subjects and in 53 (106 chromosomes) MPS-I patients.</p><p><strong><em>Results. –</em></strong> The genotypes for IDUA <em>Eco</em>47III and <em>Nsp</em>I sites in Mexicans were in agreement with Hardy–Weinberg expectations. Allele frequency distributions for individual sites differed (<em>P</em> < 0.05) in both groups. Haplotype <em>Eco</em>47III–<em>Nsp</em>I frequencies of Mexican MPS-I patients also differed from those of the normal Mexican population. The data provide evidence of linkage disequilibrium, since the MPS-I group constitutes a subset of the Mexican control population. The disequilibrium in Mexican MPS-I patients was defined by an increase in the haplotype A<sub>1</sub>B<sub>2</sub>, and deficiency in A<sub>2</sub>B<sub>1</sub>, with respect to normal population (<em>P</em> < 0.05).</p><p><strong><em>Conclusions. –</em></strong> Our results support that these polymorphisms can be associated to mutations in IDUA gene, which leads to MPS-I in Mexican patients. On the other hand, these polymorphisms can be used to identify heterozygosity when they are informative.</p></div>","PeriodicalId":100089,"journal":{"name":"Annales de Génétique","volume":"46 1","pages":"Pages 7-10"},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0003-3995(03)00010-8","citationCount":"2","resultStr":"{\"title\":\"Mucopolysaccharidosis I: a comparative study of haplotypes Eco47III–NspI sites frequencies in patients and healthy subjects of Mexican population\",\"authors\":\"M.P. Gallegos-Arreola , L. Arnaud-López , L.E. Figuera , T.S.Beltrán Jaramillo , H. Rangel-Villalobos , Sunji Thomatsu , G.M. Zúñiga-González\",\"doi\":\"10.1016/S0003-3995(03)00010-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><strong><em>Background. –</em></strong> Mucopolysaccharidosis I (MPS-I) is an autosomal recessive disorder, which is caused by mutations in the IDUA gene. It induces the deficiency of glycosidase α-L-duronidase. The enzyme that is required for the degradation of heparan and dermatan sulfate. This disorder expresses a wide range of clinical symptoms (severe mental retardation, skeletal deformations, hepatosplenomegaly, corneal clouding and mild visceral organ involvement). In the present paper, we report the frequencies of haplotypes of the <em>Eco</em>47III–<em>Nsp</em>I sites, in the IDUA gene, in Mexican healthy and in MPS-I individuals.</p><p><strong><em>Methods. –</em></strong><em>Eco</em>47III and <em>Nsp</em>I intragenic polymorphisms in IDUA gene were studied in 262 (524 chromosomes) Mexican healthy subjects and in 53 (106 chromosomes) MPS-I patients.</p><p><strong><em>Results. –</em></strong> The genotypes for IDUA <em>Eco</em>47III and <em>Nsp</em>I sites in Mexicans were in agreement with Hardy–Weinberg expectations. Allele frequency distributions for individual sites differed (<em>P</em> < 0.05) in both groups. Haplotype <em>Eco</em>47III–<em>Nsp</em>I frequencies of Mexican MPS-I patients also differed from those of the normal Mexican population. The data provide evidence of linkage disequilibrium, since the MPS-I group constitutes a subset of the Mexican control population. The disequilibrium in Mexican MPS-I patients was defined by an increase in the haplotype A<sub>1</sub>B<sub>2</sub>, and deficiency in A<sub>2</sub>B<sub>1</sub>, with respect to normal population (<em>P</em> < 0.05).</p><p><strong><em>Conclusions. –</em></strong> Our results support that these polymorphisms can be associated to mutations in IDUA gene, which leads to MPS-I in Mexican patients. 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引用次数: 2
摘要
背景。粘多糖病I (MPS-I)是一种常染色体隐性遗传病,由IDUA基因突变引起。诱导糖苷酶α- l -榴莲苷酶缺乏。肝素酶一种降解肝素和硫酸皮素所必需的酶这种疾病表现出广泛的临床症状(严重的智力迟钝、骨骼变形、肝脾肿大、角膜混浊和轻微的内脏器官受累)。在本文中,我们报告了墨西哥健康和MPS-I个体中IDUA基因中Eco47III-NspI位点的单倍型频率。对262名墨西哥健康人(524条染色体)和53名MPS-I患者(106条染色体)的IDUA基因eco47iii和NspI基因内多态性进行了研究。墨西哥人IDUA Eco47III和NspI位点的基因型与Hardy-Weinberg预期一致。个体位点的等位基因频率分布存在差异(P <0.05)。墨西哥MPS-I患者的单倍型Eco47III-NspI频率也不同于墨西哥正常人群。这些数据提供了连锁不平衡的证据,因为MPS-I组构成了墨西哥对照人群的一个子集。与正常人群相比,墨西哥MPS-I患者的不平衡表现为A1B2单倍型增加,A2B1缺乏(P <0.05) .Conclusions。我们的结果支持这些多态性可能与IDUA基因突变有关,这导致了墨西哥患者的MPS-I。另一方面,当这些多态性具有信息性时,可用于鉴定杂合性。
Mucopolysaccharidosis I: a comparative study of haplotypes Eco47III–NspI sites frequencies in patients and healthy subjects of Mexican population
Background. – Mucopolysaccharidosis I (MPS-I) is an autosomal recessive disorder, which is caused by mutations in the IDUA gene. It induces the deficiency of glycosidase α-L-duronidase. The enzyme that is required for the degradation of heparan and dermatan sulfate. This disorder expresses a wide range of clinical symptoms (severe mental retardation, skeletal deformations, hepatosplenomegaly, corneal clouding and mild visceral organ involvement). In the present paper, we report the frequencies of haplotypes of the Eco47III–NspI sites, in the IDUA gene, in Mexican healthy and in MPS-I individuals.
Methods. –Eco47III and NspI intragenic polymorphisms in IDUA gene were studied in 262 (524 chromosomes) Mexican healthy subjects and in 53 (106 chromosomes) MPS-I patients.
Results. – The genotypes for IDUA Eco47III and NspI sites in Mexicans were in agreement with Hardy–Weinberg expectations. Allele frequency distributions for individual sites differed (P < 0.05) in both groups. Haplotype Eco47III–NspI frequencies of Mexican MPS-I patients also differed from those of the normal Mexican population. The data provide evidence of linkage disequilibrium, since the MPS-I group constitutes a subset of the Mexican control population. The disequilibrium in Mexican MPS-I patients was defined by an increase in the haplotype A1B2, and deficiency in A2B1, with respect to normal population (P < 0.05).
Conclusions. – Our results support that these polymorphisms can be associated to mutations in IDUA gene, which leads to MPS-I in Mexican patients. On the other hand, these polymorphisms can be used to identify heterozygosity when they are informative.