E-cadherin和β - catenin在原发性和转移性肾母细胞瘤中的差异表达。

J Alami, B R Williams, H Yeger
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引用次数: 24

摘要

背景:e -钙粘蛋白-连环蛋白黏附复合物对细胞间黏附和组织结构的维持至关重要。它的损伤与低分化表型和癌的侵袭性增加有关。目的:探讨E-cadherin、β - catenin、γ - catenin和ezrin在原发性和转移性肾母细胞瘤中的表达及其与组织病理学特征的关系。方法:采用免疫组化方法检测E-cadherin、β - catenin、γ - catenin和ezrin在原发性和转移性Wilms肿瘤中的表达和细胞分布。Western blotting检测Wilms肿瘤中E-cadherin和β - catenin的多肽大小及表达。结果:E-cadherin主要在发育不良小管细胞质中表达,偶尔在细胞膜中表达,在胚细胞细胞质中表达较低。原发性和转移性肿瘤显示-连环蛋白在囊胚和上皮细胞中高表达,主要呈膜质和细胞质染色。转移性肿瘤偶见核染。γ -连环蛋白和ezrin在原发和转移性肿瘤的母细胞和上皮细胞的细胞质中有低到高的表达。肿瘤中检测到的92 kDa β连环蛋白含量高于正常肾脏。120 kDa E-cadherin在中度分化肿瘤中低表达,而在低分化肿瘤中缺乏表达。结论:与原发肿瘤相比,转移性肿瘤E-cadherin和γ - catenin表达较低,β - catenin核染色。低e -钙粘蛋白与低分化肿瘤相关。这些结果表明,粘附蛋白的异常表达与Wilms肿瘤的侵袭性和转移性表型相关。
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Differential expression of E-cadherin and beta catenin in primary and metastatic Wilms's tumours.

Background: The E-cadherin-catenin adhesion complex is crucial for intercellular adhesiveness and maintenance of tissue architecture. Its impairment is associated with poorly differentiated phenotype and increased invasiveness of carcinomas.

Aims: To evaluate E-cadherin, beta catenin, gamma catenin, and ezrin expression and its relation to histopathological features of primary and metastatic Wilms's tumours.

Methods: Immunohistochemistry was used to determine the expression and cellular distribution of E-cadherin, beta catenin, gamma catenin, and ezrin in primary and metastatic Wilms's tumours. Western blotting was used to determine polypeptide size and expression of E-cadherin and beta catenin in Wilms's tumours compared with normal kidney.

Results: Moderate expression of E-cadherin was found mainly in cytoplasm and occasionally cell membranes of dysplastic tubules, whereas low expression was seen in cytoplasm of blastemal cells. Primary and metastatic tumours showed moderate to high beta catenin expression in blastemal and epithelial cells, with predominantly membranous and cytoplasmic staining. Occasional nuclear staining was noted in metastatic tumours. Low to high gamma catenin and ezrin expression was seen in cytoplasm of blastemal and epithelial cells of primary and metastatic tumours. Higher amounts of 92 kDa beta catenin were detected in tumours than in normal kidney. Low expression of 120 kDa E-cadherin was seen in moderately differentiated tumours, whereas expression was lacking in poorly differentiated tumours.

Conclusions: Compared with primary tumours, metastatic tumours showed lower expression of E-cadherin and gamma catenin, with nuclear staining for beta catenin. Low E-cadherin was associated with poorly differentiated tumours. These results suggest that abnormal expression of adhesion proteins correlates with the invasive and metastatic phenotype in Wilms's tumours.

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