从RecA衍生的肽与含有5-甲酰基-2'-脱氧尿苷的单链寡核苷酸的相互作用。

Nucleic acids symposium series Pub Date : 2000-01-01 DOI:10.1093/nass/44.1.41

T Sugiyama, A Kittaka, H Takayama, M Tomioka, Y Ida, R Kuroda

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引用次数: 1

摘要

我们报告了第一个通过希夫碱形成的含有寡核苷酸的5-甲酰基-2'-脱氧尿苷与寡肽的化学交联的例子。本文研究的20个氨基酸残基肽来自RecA蛋白的DNA结合位点。我们已经证明了位于肽n端第6或第8位的赖氨酸残基直接与DNA接触。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Interaction of peptides derived from RecA with single-stranded oligonucleotides containing 5-formyl-2'-deoxyuridine.

We report the first example of chemical cross-linking of 5-formyl-2'-deoxyuridine containing oligonucleotides with oligopeptides through a Schiff base formation. Twenty amino acid residue peptides investigated here were derived from the DNA binding site of RecA protein. We have demonstrated that the lysine residue placed at the 6th or 8th position from the N-terminus of the peptide directly contacts with DNA.

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Nucleic acids symposium series

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