Ce(IV)/EDTA络合物对DNA的水解——DNA裂解机制及人工限制性内切酶的设计。

Nucleic acids symposium series Pub Date : 2000-01-01 DOI:10.1093/nass/44.1.231

Y Yamamoto, T Igawa, J Sumaoka, M Komiyama

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引用次数: 0

摘要

EDTA的均相Ce(IV)配合物在生理条件下能迅速水解寡核苷酸。此外，添加胺可以促进Ce(IV)/EDTA的DNA水解活性。当[Ce(IV)/EDTA] = 5 mmol dm3，[乙二胺]= 100 mmol dm3时，催化活性约为Ce(IV)/EDTA的50倍。Ce(IV)/EDTA与胺的结合是未来分子生物学和生物技术的重要工具。

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Hydrolysis of DNA by Ce(IV)/EDTA complexes--the mechanism of DNA cleavage and design of artificial restriction enzymes.

Homogeneous Ce(IV) complex of EDTA promptly hydrolyzes oligonucleotides under physiological conditions. Moreover, the activity of Ce(IV)/EDTA for DNA hydrolysis is promoted by the addition of amines. When [Ce(IV)/EDTA] = 5 mumol dm3 and [ethylenediamine] = 100 mmol dm3, the catalytic activity is about 50 times as large as that of Ce(IV)/EDTA. The combination of Ce(IV)/EDTA and amines is eminent tools for the future molecular biology and biotechnology.

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Nucleic acids symposium series

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