最活跃的rna切割脱氧核酶的结晶。

J Kondo, A Takénaka
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引用次数: 3

摘要

对活性最高的脱氧核酶及其衍生物的结构组成进行了电泳分析,并对其结晶条件进行了x射线分析。研究发现,Mg2+离子是催化DNA与底物之间形成活性二元配合物所必需的,热处理可以有效地防止两种酶与两种底物之间形成非活性的四元配合物。悬垂滴气扩散法得到的晶体是由活性二元配合物组成的。
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Crystallization of the most active RNA-cleaving deoxyribozyme.

The structural compositions of the most active deoxyribozyme and its derivatives have been examined by electrophoresis, and their crystallization conditions were surveyed for X-ray analysis. It has been found that Mg2+ ion is essential to form the active binary complex between the catalytic DNA and the substrate, and that heat-treatment is effective to prevent formation of the inactive quaternary complex between the two enzymes and the two substrates. Crystals obtained by the hanging drop vapor diffusion method are composed of the active binary complex.

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