一种具有短Ca(2+)依赖性脱氧核酶的新型功能性生物传感器的研制。

Nucleic acids symposium series Pub Date : 2000-01-01 DOI:10.1093/nass/44.1.79

Y Okumoto, N Sugimoto

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引用次数: 1

摘要

我们开发了一种基于脱氧核酶的新型功能性生物传感器。5'端固定化短Ca(2+)依赖性脱氧核酶(dCGCTGGCAGGCTACAACGAGTCTTC)结合靶RNA底物(rGAAGACA减少UGCCAGCG;减少表示RNA切割位点)，并在Ca2+存在下作为酶。它在不对称内环的rAp降低U的一个位点切割靶RNA底物。

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Development of a novel functional biosensor with a short Ca(2+)-dependent deoxyribozyme.

We develop a novel functional biosensor on a deoxyribozyme. A 5'-end-immobilized short Ca(2+)-dependent deoxyribozyme (dCGCTGGCAGGCTACAACGAGTCTTC) binds to a target RNA substrate (rGAAGACA decrease UGCCAGCG; decrease denotes an RNA cleavage site), and acts as an enzyme in the presence of Ca2+. It cleaves the target RNA substrate at one site of rAp decrease U in the asymmetric internal loop.

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Nucleic acids symposium series

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