5-甲酰基尿嘧啶在哺乳动物细胞中的致突变性。

Nucleic acids symposium series Pub Date : 2000-01-01 DOI:10.1093/nass/44.1.81

H Kamiya, N Murata-Kamiya, N Karino, Y Ueno, A Matsuda, H Kasai

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引用次数: 2

摘要

将胸腺嘧啶的一种主要氧化形式5-甲酰基尿嘧啶(5-甲酰基尿嘧啶)加入到双链载体的前导和滞后模板链的预定位点，研究了5-甲酰基尿嘧啶在猿猴COS-7细胞中的DNA复制效率和突变频率。5-甲酰基尿嘧啶不阻断DNA复制，在猿猴细胞中具有弱致突变性。5-甲酰基尿嘧啶主要在修饰位置引起碱基取代。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mutagenicity of 5-formyluracil in mammalian cells.

5-Formyluracil, a major oxidized form of thymine, was incorporated into a predetermined site of one of the leading and lagging template strands of a double-stranded vector, and the DNA replication efficiency and the mutation frequency of 5-formyluracil in simian COS-7 cells were investigated. 5-Formyluracil did not block DNA replication and was weakly mutagenic in simian cells. 5-Formyluracil primarily elicited base substitutions at the modified positions.

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Nucleic acids symposium series

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