异交底外源性眼肌神经肌肉连接及肌浆网的精细结构。

J F REGER
{"title":"异交底外源性眼肌神经肌肉连接及肌浆网的精细结构。","authors":"J F REGER","doi":"10.1083/jcb.10.4.111","DOIUrl":null,"url":null,"abstract":"<p><p>The extrinsic eye muscles of the killifish (F. heteroclitus) were fixed in OSO(4) (pH 7.6) and subsequently dehydrated, embedded, and sectioned for electron microscopy. The fine structures of neuromuscular junctions and of sarcoplasmic reticulum were then observed. The neuromuscular junction consists of the apposition of axolemma (60 to 70 A) and sarcolemma (90 to 100 A), with an intervening cleft space of 200 to 300 A, forming a synaptolemma 400 to 500 A thick. The terminal axons contain synaptic vesicles, mitochondria, and agranular reticulum. The subsynaptic sarcolemma lacks the infolding arrangement characteristic of neuromuscular junctions from other vertebrate skeletal muscle, making them more nearly like that of insect neuromuscular junctions. A comparison between the folded and non-folded subsynaptic membrane types is made and discussed in terms of comparative rates of acetylcholine diffusion from the synaptic cleft and resistances of the clefts and subsynaptic membranes. The sarcoplasmic reticulum consists of segmentally arranged, membrane-limited vesicles and tubular and cisternal elements which surround individual myofibrils in a sleeve-like arrangement. Triadic differentiation occurs at or near the A-I junction. Unit sleeves span the A and I bands alternately and consist of closed terminal cisternae interconnected across the A and I bands by tubular cisternae. The thickness of the sarcoplasmic membranes increases from 30 to 40 A in intertriadic regions to 50 to 70 A at the triads. The location of the triads is compared with previously described striated muscle from Ambystoma larval myotomes, cardiac and sartorius muscles of the albino rat, mouse limb muscle, chameleon lizard muscle, and insect muscle, with reference to their possible role in intracellular impulse conduction.</p>","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"10(4)Suppl ","pages":"111-21"},"PeriodicalIF":0.0000,"publicationDate":"1961-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.10.4.111","citationCount":"54","resultStr":"{\"title\":\"The fine structure of neuromuscular junctions and the sarcoplasmic reticulum of extrinsic eye muscles of Fundulus heteroclitus.\",\"authors\":\"J F REGER\",\"doi\":\"10.1083/jcb.10.4.111\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The extrinsic eye muscles of the killifish (F. heteroclitus) were fixed in OSO(4) (pH 7.6) and subsequently dehydrated, embedded, and sectioned for electron microscopy. The fine structures of neuromuscular junctions and of sarcoplasmic reticulum were then observed. The neuromuscular junction consists of the apposition of axolemma (60 to 70 A) and sarcolemma (90 to 100 A), with an intervening cleft space of 200 to 300 A, forming a synaptolemma 400 to 500 A thick. The terminal axons contain synaptic vesicles, mitochondria, and agranular reticulum. The subsynaptic sarcolemma lacks the infolding arrangement characteristic of neuromuscular junctions from other vertebrate skeletal muscle, making them more nearly like that of insect neuromuscular junctions. A comparison between the folded and non-folded subsynaptic membrane types is made and discussed in terms of comparative rates of acetylcholine diffusion from the synaptic cleft and resistances of the clefts and subsynaptic membranes. The sarcoplasmic reticulum consists of segmentally arranged, membrane-limited vesicles and tubular and cisternal elements which surround individual myofibrils in a sleeve-like arrangement. Triadic differentiation occurs at or near the A-I junction. Unit sleeves span the A and I bands alternately and consist of closed terminal cisternae interconnected across the A and I bands by tubular cisternae. The thickness of the sarcoplasmic membranes increases from 30 to 40 A in intertriadic regions to 50 to 70 A at the triads. The location of the triads is compared with previously described striated muscle from Ambystoma larval myotomes, cardiac and sartorius muscles of the albino rat, mouse limb muscle, chameleon lizard muscle, and insect muscle, with reference to their possible role in intracellular impulse conduction.</p>\",\"PeriodicalId\":22618,\"journal\":{\"name\":\"The Journal of Biophysical and Biochemical Cytology\",\"volume\":\"10(4)Suppl \",\"pages\":\"111-21\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1961-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1083/jcb.10.4.111\",\"citationCount\":\"54\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Biophysical and Biochemical Cytology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1083/jcb.10.4.111\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Biophysical and Biochemical Cytology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1083/jcb.10.4.111","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 54

摘要

将鳉鱼(F. heteroclitus)的外源眼肌固定在OSO(4) (pH 7.6)中,随后脱水、包埋并切片用于电镜观察。观察神经肌肉连接处和肌浆网的精细结构。神经肌肉连接处由轴膜(60 ~ 70a)和肌膜(90 ~ 100a)的并列组成,中间间隙为200 ~ 300a,形成400 ~ 500a厚的突触膜。末端轴突包含突触囊泡、线粒体和颗粒网。突触下肌膜缺乏其他脊椎动物骨骼肌神经肌肉连接的内折叠排列特征,使它们更接近于昆虫的神经肌肉连接。从乙酰胆碱从突触间隙扩散的比较速率和突触间隙和亚突触膜的阻力方面,对折叠和非折叠的突触亚膜类型进行了比较和讨论。肌浆网由节段排列、膜限制的囊泡、管状和池状元件组成,它们以套筒状排列围绕着单个肌原纤维。三联分化发生在A-I交界处或附近。单元套筒交替跨越A和I波段,由封闭的终端蓄水池组成,通过管状蓄水池在A和I波段相互连接。肌质膜厚度从三合一区30 ~ 40a增加到三合一区50 ~ 70a。将三合体的位置与先前描述的Ambystoma幼虫的横纹肌、白化大鼠的心肌和缝口肌、小鼠肢体肌肉、变色龙蜥蜴肌肉和昆虫肌肉的横纹肌进行比较,以参考它们在细胞内冲动传导中的可能作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The fine structure of neuromuscular junctions and the sarcoplasmic reticulum of extrinsic eye muscles of Fundulus heteroclitus.

The extrinsic eye muscles of the killifish (F. heteroclitus) were fixed in OSO(4) (pH 7.6) and subsequently dehydrated, embedded, and sectioned for electron microscopy. The fine structures of neuromuscular junctions and of sarcoplasmic reticulum were then observed. The neuromuscular junction consists of the apposition of axolemma (60 to 70 A) and sarcolemma (90 to 100 A), with an intervening cleft space of 200 to 300 A, forming a synaptolemma 400 to 500 A thick. The terminal axons contain synaptic vesicles, mitochondria, and agranular reticulum. The subsynaptic sarcolemma lacks the infolding arrangement characteristic of neuromuscular junctions from other vertebrate skeletal muscle, making them more nearly like that of insect neuromuscular junctions. A comparison between the folded and non-folded subsynaptic membrane types is made and discussed in terms of comparative rates of acetylcholine diffusion from the synaptic cleft and resistances of the clefts and subsynaptic membranes. The sarcoplasmic reticulum consists of segmentally arranged, membrane-limited vesicles and tubular and cisternal elements which surround individual myofibrils in a sleeve-like arrangement. Triadic differentiation occurs at or near the A-I junction. Unit sleeves span the A and I bands alternately and consist of closed terminal cisternae interconnected across the A and I bands by tubular cisternae. The thickness of the sarcoplasmic membranes increases from 30 to 40 A in intertriadic regions to 50 to 70 A at the triads. The location of the triads is compared with previously described striated muscle from Ambystoma larval myotomes, cardiac and sartorius muscles of the albino rat, mouse limb muscle, chameleon lizard muscle, and insect muscle, with reference to their possible role in intracellular impulse conduction.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Histochemistry of the centrioles and centrosomes of the leukemic cells from human myeloblastic leukemia. The ultrastructure and histochemistry of a nematode-induced giant cell. The intracellular distribution and heterogeneity of ribonucleic acid in starfish oocytes. A small granular component of the cytoplasm of keratinizing epithelia. Corpus intra cristam: a dense body within mitochondria of cells in hyperplastic mouse epidermis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1