线虫诱导的巨细胞的超微结构和组织化学。

A F BIRD
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引用次数: 160

摘要

在控制生长条件下,观察了番茄根内巨细胞的发育过程,并对巨细胞的超微结构和组织化学进行了研究。线虫幼虫在24小时内进入根部;巨细胞形成开始于第4天,包括细胞壁的破坏,伴随着周围巨细胞壁的增厚,细胞质的密度和面积增加。在单个巨细胞中,细胞核通过同时有丝分裂而增加。细胞质密度在蜕皮后和产蛋期间达到峰值。巨细胞中蛋白质合成的速率与线虫的生长速率相关。巨细胞壁是一种厚的、不规则的表面结构,它包含细胞壁的所有正常的多糖成分。细胞质富含蛋白质和RNA,含有线粒体、前质体、高尔基体和致密的内质网。细胞核大而不规则,含有大核仁和沿核膜不规则分布的许多feulgen阳性小体。核仁含有RNA和脂肪,以及核糖核酸酶处理后显示的feulgen阳性颗粒。它由致密的外皮层围绕着较轻的中央核组成,有时与核中的feulgen阳性体相连。对这些小体在细胞质蛋白质合成中的作用进行了推测。
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The ultrastructure and histochemistry of a nematode-induced giant cell.

The development of giant cells induced by the nematode Meloidogyne in tomato roots has been followed under controlled growth conditions and the ultrastructure and histochemistry of these structures have been examined. Entry of the nematode larvae into the roots took place within 24 hours; giant cell formation started on the 4th day and involved breakdown of the cell walls accompanied by thickening of a surrounding giant cell wall and an increase in density and area of the cytoplasm. The nuclei increased in number by simultaneous mitosis throughout a single giant cell. The peak of cytoplasmic density was reached after moulting and during egg production. The rate of protein synthesis in the giant cell is correlated with the rate of growth of the nematode. The giant cell wall is a thick, irregularly surfaced structure which contains all the normal polysaccharide components of a cell wall. The cytoplasm is rich in protein and RNA and contains mitochondria, proplastids, Golgi bodies, and a dense endoplasmic reticulum. The nuclei are large and irregular in shape and contain large nucleoli and a number of Feulgen-positive bodies scattered irregularly along the nuclear envelope. The nucleolus contains RNA and fat as well as Feulgen-positive granules which are revealed after treatment with ribonuclease. It consists of a dense outer cortex surrounding a much lighter central core and is connected at times with the Feulgen-positive bodies in the nucleus. Speculation is provided on the role of these bodies in cytoplasmic protein synthesis.

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