{"title":"丙酮酸盐与糖精梭状芽胞杆菌的磷碎裂反应表明丙酮酸盐降解中有一种翼状辅助因子的作用","authors":"Colette Delavier-Klutchko","doi":"10.1016/0006-3002(63)91022-9","DOIUrl":null,"url":null,"abstract":"<div><p><em>Clostridium saccharobutyricum</em> extracts, heat treated at 65° have somewhat similar properties to iron-deficient extracts prepared from this organism: they cannot degrade pyruvate by the phosphoroclastic pathway or incorporate <sup>14</sup>CO<sub>2</sub> into pyruvate. However, they stimulate the clastic activity of normal extracts and restore the clastic activity of aged extracts. A mixture of heat-treated (65°) and iron-deficient extracts is inactive. The heat-treated (65°) extract has no stimulating effect on the <sup>14</sup>CO<sub>2</sub>-pyruvate exchange reaction. A cofactor FCL, of the formylfolic acid type was isolated from at 65° treated extracts. It increases the rat off oxidation of pyruvate. <em>N</em><sup>10</sup>-formyltetrahydrofolic acid replaces FCL whereas folic, <em>N</em><sup>10</sup>-formylfolic and <em>N</em><sup>5</sup>-formyltetrahydrofolic acids are inactive.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1963-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91022-9","citationCount":"1","resultStr":"{\"title\":\"Réaction phosphoroclastique du pyruvate par Clostridium saccharobutyricum Mise en évidence de l'intervention d'un cofacteur de nature ptéridique dans la dégradation du pyruvate\",\"authors\":\"Colette Delavier-Klutchko\",\"doi\":\"10.1016/0006-3002(63)91022-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Clostridium saccharobutyricum</em> extracts, heat treated at 65° have somewhat similar properties to iron-deficient extracts prepared from this organism: they cannot degrade pyruvate by the phosphoroclastic pathway or incorporate <sup>14</sup>CO<sub>2</sub> into pyruvate. However, they stimulate the clastic activity of normal extracts and restore the clastic activity of aged extracts. A mixture of heat-treated (65°) and iron-deficient extracts is inactive. The heat-treated (65°) extract has no stimulating effect on the <sup>14</sup>CO<sub>2</sub>-pyruvate exchange reaction. A cofactor FCL, of the formylfolic acid type was isolated from at 65° treated extracts. It increases the rat off oxidation of pyruvate. <em>N</em><sup>10</sup>-formyltetrahydrofolic acid replaces FCL whereas folic, <em>N</em><sup>10</sup>-formylfolic and <em>N</em><sup>5</sup>-formyltetrahydrofolic acids are inactive.</p></div>\",\"PeriodicalId\":94301,\"journal\":{\"name\":\"Biochimica et biophysica acta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1963-12-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0006-3002(63)91022-9\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et biophysica acta\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0006300263910229\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et biophysica acta","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0006300263910229","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Réaction phosphoroclastique du pyruvate par Clostridium saccharobutyricum Mise en évidence de l'intervention d'un cofacteur de nature ptéridique dans la dégradation du pyruvate
Clostridium saccharobutyricum extracts, heat treated at 65° have somewhat similar properties to iron-deficient extracts prepared from this organism: they cannot degrade pyruvate by the phosphoroclastic pathway or incorporate 14CO2 into pyruvate. However, they stimulate the clastic activity of normal extracts and restore the clastic activity of aged extracts. A mixture of heat-treated (65°) and iron-deficient extracts is inactive. The heat-treated (65°) extract has no stimulating effect on the 14CO2-pyruvate exchange reaction. A cofactor FCL, of the formylfolic acid type was isolated from at 65° treated extracts. It increases the rat off oxidation of pyruvate. N10-formyltetrahydrofolic acid replaces FCL whereas folic, N10-formylfolic and N5-formyltetrahydrofolic acids are inactive.
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