On the lack of a uniform specificity of aminoacyl ribonucleic acid synthetases from different organisms

11 Aminoacyl RNA's of Escherichia coli were prepared using the homologous (E. coli) aminoacyl RNA synthetases (amino acid-RNA ligases). Their chromatographic behavior was compared to those aminoacyl RNA's prepared using three heterologous (mouse liver, maize, Neurospora crassa) synthetases. In some cases the products of two enzyme preparations with a single amino acid and a single s-RNA mixture are chromatographically separable, indicating a chemical difference in the s-RNA molecules selected by the enzymes from different sources.

The relative order of chromatographic mobilities of the 11 aminoacyl RNA's from each organism, homologously aminoacylated, were shown to vary.