光合电子传递系统与红红螺旋藻染色质磷酸化耦联的位点

T. Horio, J. Yamashita
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引用次数: 29

摘要

1.1. 利用红红螺旋藻的染色质,研究了磷酸化与光合电子传递系统的耦合作用。在固定的高强度白光照射下,需要添加严格限定浓度的苯那嗪,以最大速率诱导光磷酸化。甲氧基磺酸非那嗪诱导的最大光磷酸化速率总是比抗坏血酸盐或琥珀酸盐诱导的快得多。不同批次的染色质制剂中,甲氨硫非那嗪诱导的最大速率与抗坏血酸诱导的最大速率之比不同,实验值为2 ~ 5.3.3。通过紫外照射和添加以下抑制剂:Na2SO4、盐酸醌、2,4-二硝基苯酚、双氨基酚EDTA和醋酸苯汞,以类似的方式灭活了甲氨硫肼诱导的和抗坏血酸诱导的光磷酸化。如果共振或加热,染色质悬浮液失去抗坏血酸诱导的光磷酸化活性比非那嗪甲硫代盐诱导的光磷酸化活性更快。在适当浓度的邻菲罗啉存在下,抗坏血酸诱导的光磷酸化和琥珀酸诱导的光磷酸化完全失活,但苯那嗪甲磺酸诱导的光磷酸化不受影响。在适当浓度的Triton X-100处理下,抗坏血酸诱导的光磷酸化和琥珀酸诱导的光磷酸化完全失活,而非那嗪甲硫代盐诱导的光磷酸化仅部分失活。ADP的加入加速了细胞色素c2和细胞色素c的光氧化。在Pi存在或不存在的情况下,ADP的刺激没有显著影响。在ADP不存在的情况下,2,4-二硝基苯酚促进了ADP的光氧化作用。刺激所需的2,4-二硝基苯酚浓度与抑制光磷酸化所需的浓度几乎相同。由ADP和Pi合成ATP,再加上细胞色素c2和细胞色素c的光氧化,得到的P/O值小于1。基于这些发现,提出了磷酸化与光合电子传递系统耦合的位点。
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Site of photosynthetic electron-transport systems coupling phosphorylation with chromatophores from Rhodospirillum rubrum

  • 1.

    1. With chromatophores from Rhodospirillum rubrum, phosphorylations coupled with photosynthetic electron-transport systems have been investigated.

  • 2.

    2. Under illumination with white light at a fixed high intensity, the addition of a strictly limited concentration of phenazine methosulfate was required to induce photophosphorylation at a maximal rate. The maximal rate of photophosphorylation was always much faster when induced by phenazine methosulfate than when induced by the other reagents tested such as ascorbate or succinate. The ratio of maximal rate of the phenazine methosulfate-induced to ascorbate-induced photophosphorylation varied from batch to batch of chromatophore preparations with experimental values from 2 to 5.

  • 3.

    3. The phenazine methosulfate-induced, and ascorbate-induced photophosphorylations were inactivated in a similar manner by illumination of chromatophores with ultraviolet irradiation and by additions of the following inhibitors: Na2SO4, quinacrine hydrochloride, 2,4-dinitrophenol, dicumarol EDTA and phenylmercuric acetate. Chromatophore suspensions, if resonicated or heated, lost the activity of ascorbate-induced photophosphorylation somewhat more rapidly than that of phenazine methosulfate-induced photophosphorylation. In the presence of an appropriate concentration of o-phenanthroline, the ascorbate-induced and succinate-induced photophosphorylations were inactivated completely, but the phenazine methosulfate-induced photophosphorylation was not affected. With the chromatophores treated with an appropriate concentration of Triton X-100, the ascorbate-induced and succinate-induced photophosphorylations were inactivated completely, whereas the phenazine methosulfate-induced photophosphorylation was inactivated only partially.

  • 4.

    4. The photooxidations of cytochrome c2, and cytochrome c were stimulated in rate by the addition of ADP. The stimulation by ADP was not significantly influenced in the presence or absence of Pi. The photooxidation was stimulated by 2,4-dinitrophenol in the absence but not in the presence of ADP. The concentraton of 2,4-dinitrophenol required for the stimulation was nearly identical with that required for the inhibition of photophosphorylation.

  • 5.

    5. ATP was synthesized from ADP and Pi, coupled with the photooxidations of cytochrome c2, and cytochrome c. The values of the P/O ratio obtained were less than one.

  • 6.

    6. Based on these findings, the site for phosphorylation coupled with the photosynthetic electron-transport systems are suggested.

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Subject index Author index Photoreduction of nicotinamide-adenine dinucleotide by a cell-free system from Chromatium Chlorophylls of photosynthetic bacteria Photodimerization of urocanic acid in vitro and in vivo
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