又是一种新的植物蛋白酶

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-11-22 DOI:10.1016/0926-6569(64)90192-0

K.F. Tipton

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引用次数: 8

摘要

1.1. 描述了从粗剑麻提取物中分离结晶酶的方法。2.2。通过电泳、deae -纤维素层析、Sephadex凝胶过滤和溶解度证明该结晶酶是均匀的。结晶酶在溶液中是不稳定的，会自发地变成一种分子量较小的无活性蛋白质。通过deae纤维素粗酶层析得到的结晶酶与F组分的一致性已得到证实。酶的分子量已测定。这种酶被称为agavian。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Agavain: A new plant proteinase

1.
1. A procedure is described for the isolation of a crystalline enzyme from crude sisal extract.
2.
2. The crystalline enzyme has been shown to be homogeneous by electrophoresis, chromatography on DEAE-cellulose, gel filtration on Sephadex, and solubility.
3.
3. The crystalline enzyme is unstable in solution, changing spontaneously to an inactive protein of smaller molecular weight.
4.
4. The identity of the crystalline enzyme with Fraction F obtained by chromatography of the crude enzyme of DEAE-cellulose has been demonstrated.
5.
5. The molecular weight of the enzyme has been determined.
6.
6. The trivial name agavian has been adopted for the enzyme.

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