Pseudomonas aeruginosa peptide peptidohydrolase IV. Optical rotatory dispersion and amino acid composition

Some physico-chemical properties of the crystalline proteinase (peptide peptidohydrolase) from Pseudomonas aeruginosa IFO 3080 were studied. The [α]_D was −30°. The λ_c was 227.6 mμ and b₀ was −30, derived from the optical rotatory dispersion. The helical content was calculated as 5.2% from the value of b₀. The amino acid composition of the proteinase was as follows: aspartic acid 66, threonine 24, serine 42, glutamic acid 35, proline 11, glycine 65, alanine 58, valine 25, isoleucine 17, leucine 37, tyrosine 21, phenylalanine 20, lysine 16, histidine 6, arginine 7, tryptophan 6. Amino acids containing sulfur, such as cystine, cysteine and methionine, were not detected.