{"title":"埃利希腹水癌细胞表面及细胞质膜的研究","authors":"Donald F. Hoelzl Wallach, Donna Ullrey","doi":"10.1016/0926-6577(64)90104-4","DOIUrl":null,"url":null,"abstract":"<div><p>A membrane fraction isolated from Ehrlich ascites carcinoma microsomes contians an ATP phosphohydrolas which is strongly stimulated by K<sup>+</sup> in the presence of Na<sup>+</sup>. The enzyme system has an absolute requirement for Na<sup>+</sup> and Mg<sup>2+</sup>, but a number of monovalent cations can simulate K<sup>+</sup>. Ca<sup>2+</sup>, ADP and ouabain are inhibitory. The enzyme is believed to be associated with plasma membrane fragments.</p><p>The quantitative relationships between enzyme activity and reactant concentration is presented and the probable relationship between ion transport in the whole cell and the alkali-cation-sensitive ATP phosphohydrolase is discussed.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 620-629"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90104-4","citationCount":"35","resultStr":"{\"title\":\"Studies on the surface and cytoplasmic membranes of Ehrlich ascites carcinoma cells\",\"authors\":\"Donald F. Hoelzl Wallach, Donna Ullrey\",\"doi\":\"10.1016/0926-6577(64)90104-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A membrane fraction isolated from Ehrlich ascites carcinoma microsomes contians an ATP phosphohydrolas which is strongly stimulated by K<sup>+</sup> in the presence of Na<sup>+</sup>. The enzyme system has an absolute requirement for Na<sup>+</sup> and Mg<sup>2+</sup>, but a number of monovalent cations can simulate K<sup>+</sup>. Ca<sup>2+</sup>, ADP and ouabain are inhibitory. The enzyme is believed to be associated with plasma membrane fragments.</p><p>The quantitative relationships between enzyme activity and reactant concentration is presented and the probable relationship between ion transport in the whole cell and the alkali-cation-sensitive ATP phosphohydrolase is discussed.</p></div>\",\"PeriodicalId\":100169,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"volume\":\"88 3\",\"pages\":\"Pages 620-629\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-11-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6577(64)90104-4\",\"citationCount\":\"35\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926657764901044\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926657764901044","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Studies on the surface and cytoplasmic membranes of Ehrlich ascites carcinoma cells
A membrane fraction isolated from Ehrlich ascites carcinoma microsomes contians an ATP phosphohydrolas which is strongly stimulated by K+ in the presence of Na+. The enzyme system has an absolute requirement for Na+ and Mg2+, but a number of monovalent cations can simulate K+. Ca2+, ADP and ouabain are inhibitory. The enzyme is believed to be associated with plasma membrane fragments.
The quantitative relationships between enzyme activity and reactant concentration is presented and the probable relationship between ion transport in the whole cell and the alkali-cation-sensitive ATP phosphohydrolase is discussed.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
