硫醇对牛肾尿酸氧化酶提取及活性的影响

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-11-22 DOI:10.1016/0926-6569(64)90185-3

R. Truscoe

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引用次数: 0

摘要

1.1. 用0.2 M Tris (pH 10.5)从氧肾丙酮粉中提取尿酸氧化酶(urate:O2 oxidoreductase, EC 1.7.3.3)，通过添加一定数量的硫醇，在最佳浓度约为8 mM.2.2时，其产率提高了1.5 ~ 10倍。这些硫醇根据其对尿酸氧化酶提取物的作用可分为三类:(i)巯基乙酸、BAL、巯基乙醇、青霉胺和乙二硫醇;(i)巯基乙酸、BAL、巯基乙醇、青霉胺和乙二硫醇:对尿酸氧化酶活性无影响;(ii)半胱氨酸:随着浓度的增加，抑制作用增强，可达32 mM;(iii)谷胱甘肽:增强至8 mM;GSSG没有效果。

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Effect of thiols on extraction and activity of ox-kidney urate oxidase

1.
1. The yield of urate oxidase (urate:O₂ oxidoreductase, EC 1.7.3.3) extracted from ox-kidney acetone powder by 0.2 M Tris (pH 10.5), was raised 1.5- to 10-fold by addition of a number of thiols, at optimum concentrations of about 8 mM.
2.
2. These thiols fell into three groups, according to their action on urate oxidase extracts: (i) thioglycollic acid, BAL, mercaptoethanol, penicillamine, and ethanedithiol: (i) thioglycollic acid, BAL, mercaptoethanol, penicillamine, and etahnedithiol: no effect on urate oxidase activity; (ii) cysteine: increasing inhibition with rising concentration, up to 32 mM; (iii) GSH: increasing potentiation up to 8 mM; GSSG had no effect.

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