{"title":"细菌鞭毛的低角x射线衍射研究","authors":"G. Swanbeck, B. Forslind","doi":"10.1016/0926-6577(64)90198-6","DOIUrl":null,"url":null,"abstract":"<div><p>In order to differentiate between the various proposed models for the structure bacterial flagella, low-angle X-ray diffraction studies have been carried out on wet specimens of <em>Proteus vulgaris</em>. The low-angle equatorial diffraction pattern has been obtained and compared with the theoretically calculated patterns for a rod model and the double and triple helical structures according to <span>Labaw and Mosley</span>. The comparison shows that a model comprised of helical filaments is the most likely structure. Fourier-Bessel transformation of the experimental scattering data has been performed and the radial density-distribution curve gives low values in the middle of the structure, suggesting the flagellum has a hollow center, thus favouring the triple helical form.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 2","pages":"Pages 422-429"},"PeriodicalIF":0.0000,"publicationDate":"1964-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90198-6","citationCount":"14","resultStr":"{\"title\":\"A low-angle X-ray diffraction study of bacterial flagella\",\"authors\":\"G. Swanbeck, B. Forslind\",\"doi\":\"10.1016/0926-6577(64)90198-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In order to differentiate between the various proposed models for the structure bacterial flagella, low-angle X-ray diffraction studies have been carried out on wet specimens of <em>Proteus vulgaris</em>. The low-angle equatorial diffraction pattern has been obtained and compared with the theoretically calculated patterns for a rod model and the double and triple helical structures according to <span>Labaw and Mosley</span>. The comparison shows that a model comprised of helical filaments is the most likely structure. Fourier-Bessel transformation of the experimental scattering data has been performed and the radial density-distribution curve gives low values in the middle of the structure, suggesting the flagellum has a hollow center, thus favouring the triple helical form.</p></div>\",\"PeriodicalId\":100169,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"volume\":\"88 2\",\"pages\":\"Pages 422-429\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6577(64)90198-6\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926657764901986\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926657764901986","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A low-angle X-ray diffraction study of bacterial flagella
In order to differentiate between the various proposed models for the structure bacterial flagella, low-angle X-ray diffraction studies have been carried out on wet specimens of Proteus vulgaris. The low-angle equatorial diffraction pattern has been obtained and compared with the theoretically calculated patterns for a rod model and the double and triple helical structures according to Labaw and Mosley. The comparison shows that a model comprised of helical filaments is the most likely structure. Fourier-Bessel transformation of the experimental scattering data has been performed and the radial density-distribution curve gives low values in the middle of the structure, suggesting the flagellum has a hollow center, thus favouring the triple helical form.