{"title":"大肠杆菌中氨基酰基rna合成酶的分离和部分纯化","authors":"D.J. McCorquodale","doi":"10.1016/0926-6550(64)90001-5","DOIUrl":null,"url":null,"abstract":"<div><p>A simple method for the separation and partial purification of aminoacyl-RNA synthetases (aminoacid-RNA ligases, EC class 6.1.1) specific for tryptophan, tyrosine, methionine, isoleucine, cysteine, valine and leucine from <em>Escherichia coli</em> is described. The separations are achieved by chromatography of particle-free bacterial extracts on substituted polysaccharide resins.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90001-5","citationCount":"8","resultStr":"{\"title\":\"The separation and partial purification of aminoacyl-RNA synthetases from Escherichia coli\",\"authors\":\"D.J. McCorquodale\",\"doi\":\"10.1016/0926-6550(64)90001-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A simple method for the separation and partial purification of aminoacyl-RNA synthetases (aminoacid-RNA ligases, EC class 6.1.1) specific for tryptophan, tyrosine, methionine, isoleucine, cysteine, valine and leucine from <em>Escherichia coli</em> is described. The separations are achieved by chromatography of particle-free bacterial extracts on substituted polysaccharide resins.</p></div>\",\"PeriodicalId\":100173,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-12-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6550(64)90001-5\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926655064900015\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064900015","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The separation and partial purification of aminoacyl-RNA synthetases from Escherichia coli
A simple method for the separation and partial purification of aminoacyl-RNA synthetases (aminoacid-RNA ligases, EC class 6.1.1) specific for tryptophan, tyrosine, methionine, isoleucine, cysteine, valine and leucine from Escherichia coli is described. The separations are achieved by chromatography of particle-free bacterial extracts on substituted polysaccharide resins.
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