牛心脏线粒体洋地黄苷片段中富马酸对NADH氧化的偶联磷酸化

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-12-23 DOI:10.1016/0926-6569(64)90002-1

Darrell W. Haas

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引用次数: 48

摘要

1.1. 富马酸对NADH的氧化已经在牛心脏线粒体的完整片段和洋地黄苷片段中得到证实，这种氧化伴随着ADP.2.2的磷酸化。完整线粒体和洋地黄苷片段的P:2e比值分别为0.8和0.7。低浓度的抗霉素和2-n-庚基-4-羟基喹啉-n-氧化物使P:2e比增加0.1，而较高浓度的这些抑制剂则降低ATP的合成。在磷酸受体之前加入己基胍抑制磷酸化反应，而Synthalin(十亚甲基二胍)和苯乙基二胍对P:2e比都没有明显的影响。

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Phosphorylation coupled to the oxidation of NADH by fumarate in digitonin fragments of beef-heart mitochondria

1.
1. The oxidation of NADH by fumarate has been demonstrated in both intact and digitonin fragments of beef-heart mitochondria and this oxidation is accompanied by a concomitant phosphorylation of ADP.
2.
2. P:2e ratios of 0.8 and 0.7 have been shown for the intact mitochondria and digitonin fragments, respectively.
3.
3. Low concentrations of antimycin and 2-n-heptyl-4-hydroxyquinoline-N-oxide increase the P:2e ratios by 0.1 while higher concentrations of these inhibitors decrease the ATP synthesis.
4.
4. Hexylguanidine inhibits the phosphorylation reaction when added prior to phosphate acceptor whereas neither Synthalin (decamethylene diguanidine) nor phenylethylbiguanide have any appreciable effect on the P:2e ratio.

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