Studies on glucose-6-phosphatase and glutaminase in rat liver and kidney

All treatments which increased liver glucose-6-phosphatase (d-glucose-6-phosphate phosphohydrolase, EC 3.1.3.9) activity also caused an increase in the glucose-6-phosphatase activity of the kidney cortex. Adrenalectomy and hypophysectomy both caused marked decreases in liver and kidney-cortex glucose-6-phosphatase activities. Three treatments increased liver glutaminase (l-glutamine amidohydrolase, EC 3.5.1.2) activity; however, only one of these, a high-protein diet, increased kidney glutaminase activity. Fasting decreased both total available liver glutaminase and kidney glutaminase activities. The removal of the adrenals or pituitary caused large decreases in glutaminase activity in both the liven and cidney. It appears that factors which affected the activity of liver glucose-6-phosphatase activity had a similar effect on kidney-cortex activity. The correlations were more difficult with the glutaminase activity since alterations of glutaminase activity were not noted with as many treatments as affected glucose-6-phosphatase activity.