高效液相色谱法测定血浆样品中醋酸环丙孕酮的含量。

Bollettino chimico farmaceutico Pub Date : 2003-06-01

A Zarghi, S Dadashzadeh, A Asgari

{"title":"高效液相色谱法测定血浆样品中醋酸环丙孕酮的含量。","authors":"A Zarghi, S Dadashzadeh, A Asgari","doi":"","DOIUrl":null,"url":null,"abstract":"A rapid and sensitive high-performance liquid chromatographic method for the determination of cyproterone acetate in human plasma has been developed. The chromatographic separation was performed on an analytical mbondapak C8 column (125 yen 4.6 mm, i.d) with an isocratic mobile phase consisting of methanol-water (62.38 v/v). Using ultra violet detection at 282 nm, the detection limit for cyproterone acetate in plasma was 10 ng/ml. The calibration curve was linear over the concentration range 50-160. 0 ng/ml. Cyproterone was isolated from plasma by liquid-liquid extraction and the recovery was about 90% for plasma. The inter-day and intra-day assay coefficients of variation were found to be less than 10%.","PeriodicalId":9085,"journal":{"name":"Bollettino chimico farmaceutico","volume":"142 5","pages":"220-2"},"PeriodicalIF":0.0000,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determination of cyproterone acetate in plasma samples by high-performance liquid chromatography.\",\"authors\":\"A Zarghi, S Dadashzadeh, A Asgari\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A rapid and sensitive high-performance liquid chromatographic method for the determination of cyproterone acetate in human plasma has been developed. The chromatographic separation was performed on an analytical mbondapak C8 column (125 yen 4.6 mm, i.d) with an isocratic mobile phase consisting of methanol-water (62.38 v/v). Using ultra violet detection at 282 nm, the detection limit for cyproterone acetate in plasma was 10 ng/ml. The calibration curve was linear over the concentration range 50-160. 0 ng/ml. Cyproterone was isolated from plasma by liquid-liquid extraction and the recovery was about 90% for plasma. The inter-day and intra-day assay coefficients of variation were found to be less than 10%.\",\"PeriodicalId\":9085,\"journal\":{\"name\":\"Bollettino chimico farmaceutico\",\"volume\":\"142 5\",\"pages\":\"220-2\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bollettino chimico farmaceutico\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bollettino chimico farmaceutico","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

建立了快速、灵敏的高效液相色谱法测定人血浆中醋酸环丙孕酮的方法。色谱柱为mbondapak C8 (125 yen 4.6 mm, id)，流动相为甲醇-水(62.38 v/v)。采用282nm紫外检测，血浆中醋酸环丙孕酮的检出限为10 ng/ml。在50 ~ 160浓度范围内，校准曲线呈线性。0 ng / ml。采用液-液萃取法从血浆中分离出环丙孕酮，血浆回收率约为90%。日间和日间测定变异系数均小于10%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Determination of cyproterone acetate in plasma samples by high-performance liquid chromatography.

A rapid and sensitive high-performance liquid chromatographic method for the determination of cyproterone acetate in human plasma has been developed. The chromatographic separation was performed on an analytical mbondapak C8 column (125 yen 4.6 mm, i.d) with an isocratic mobile phase consisting of methanol-water (62.38 v/v). Using ultra violet detection at 282 nm, the detection limit for cyproterone acetate in plasma was 10 ng/ml. The calibration curve was linear over the concentration range 50-160. 0 ng/ml. Cyproterone was isolated from plasma by liquid-liquid extraction and the recovery was about 90% for plasma. The inter-day and intra-day assay coefficients of variation were found to be less than 10%.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助