硫酸Acharan,一种新的糖胺聚糖,来自Bowdich 1822。结构异质性,代谢标记和定位在体内,粘液和有机壳基质。

Tuane C R G Vieira, Adilson Costa-Filho, Norma C Salgado, Silvana Allodi, Ana-Paula Valente, Luiz E Nasciutti, Luiz-Claudio F Silva
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引用次数: 43

摘要

硫酸阿查兰(Acharan sulfate)是一种新发现的糖胺聚糖,其主要双糖重复单元为->4)-2-乙酰基,2-脱氧-- d-葡萄糖吡喃糖(1- >4)-2-磺基-- l-异吡喃氨基醛酸(1- >),与肝素和硫酸阿查兰在结构上均有亲缘关系。这种糖胺聚糖是多分散的,平均分子质量为29 kDa,已知的少量双糖序列变体含有未酸化的伊杜醛酸。用阿利新蓝染色法发现硫酸阿查兰位于该物种的体内,它被认为是粘液的主要成分。在本工作中,我们进一步研究了硫酸阿查兰在蜗牛体内的结构和区室分布。从整个身体、粘液和有机壳基质中分离出不同的硫酸阿查兰种群,它们表现出电荷和/或分子质量的异质性。此外,还从蜗牛体内纯化出易被亚硝酸降解的少量糖胺聚糖,表明存在n -硫酸化的糖胺聚糖分子。此外,我们证明了在添加了[35S]游离硫酸盐的膳食后,硫酸阿查兰在蜗牛体内的代谢标记。该方法可应用于硫酸阿查兰生物合成的研究。最后,我们开发了组织化学分析方法,通过异色染色和用[35S]硫酸盐代谢性放射性标记后的组织放射自显影来定位螺体内的硫酸阿卡兰。结果表明,硫酸阿查兰广泛分布于几个器官中。
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Acharan sulfate, the new glycosaminoglycan from Achatina fulica Bowdich 1822. Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix.

Acharan sulfate, a recently discovered glycosaminoglycan isolated from Achatina fulica, has a major disaccharide repeating unit of -->4)-2-acetyl,2-deoxy-alpha-d-glucopyranose(1-->4)-2-sulfo-alpha-l-idopyranosyluronic acid (1-->, making it structurally related to both heparin and heparan sulfate. It has been suggested that this glycosaminoglycan is polydisperse, with an average molecular mass of 29 kDa and known minor disaccharide sequence variants containing unsulfated iduronic acid. Acharan sulfate was found to be located in the body of this species using alcian blue staining and it was suggested to be the main constituent of the mucus. In the present work, we provide further information on the structure and compartmental distribution of acharan sulfate in the snail body. Different populations of acharan sulfate presenting charge and/or molecular mass heterogeneities were isolated from the whole body, as well as from mucus and from the organic shell matrix. A minor glycosaminoglycan fraction susceptible to degradation by nitrous acid was also purified from the snail body, suggesting the presence of N-sulfated glycosaminoglycan molecules. In addition, we demonstrate the in vivo metabolic labeling of acharan sulfate in the snail body after a meal supplemented with [35S]free sulfate. This simple approach might be applied to the study of acharan sulfate biosynthesis. Finally, we developed histochemical assays to localize acharan sulfate in the snail body by metachromatic staining and by histoautoradiography following metabolic radiolabeling with [35S]sulfate. Our results show that acharan sulfate is widely distributed among several organs.

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