2′-脱氧异鸟苷对人肿瘤及正常细胞系生长的影响。

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Radoslawa Nowak, Maria Sawardo-Rochowska, Zygmunt Kazimierczuk, Roman J Nowak

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摘要

目的:研究HIV逆转录酶特异性底物2′-脱氧异鸟苷对培养的正常细胞和癌细胞生长的影响。方法:观察2′-脱氧异鸟苷对2种正常(端粒酶阴性)细胞和5种癌细胞生长的影响。MTT法测定细胞活力。结果:该核苷类似物抑制肿瘤细胞生长的效力和特异性较低。肿瘤细胞的IC50范围为0.5 mM ~ 2mm，正常细胞的IC50范围为1mm ~ 4mm以上。结论:2′-脱氧异鸟苷抑制肿瘤细胞生长的效力和特异性较低，与其他端粒酶抑制剂相似。

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Effect of 2'-deoxyisoguanosine on the growth of human tumor and normal cell lines.

Aim: To test the effect of 2'-deoxyisoguanosine as a specific substrate of HIV reverse transcriptase on the growth of cultured normal and cancer cells.

Methods: The effect of 2'-deoxyisoguanosine on the growth of cells of two normal (telomerase-negative) and five cancer cell lines have been tested. Cell viability was assessed by MTT assay.

Results: We have found that this nucleoside analogue has low potency and specificity in inhibiting tumor cell growth. IC50 ranged from 0.5 mM to 2 mM for tumor cells, and from 1 mM to more than 4 mM for normal cells.

Conclusions: 2'-deoxyisoguanosine has low potency and specificity in inhibiting tumor cell growth, similar to other telomerase inhibitors.

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Eksperimental'naia onkologiia

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