Rosely M. Zancopé-Oliveira, Patrícia Morais e Silva Tavares, Mauro de Medeiros Muniz
{"title":"巴西荚膜组织浆体菌株的遗传多样性","authors":"Rosely M. Zancopé-Oliveira, Patrícia Morais e Silva Tavares, Mauro de Medeiros Muniz","doi":"10.1016/j.femsim.2005.05.018","DOIUrl":null,"url":null,"abstract":"<div><p>This study establishes the genetic relatedness among Brazilian <span><em>Histoplasma capsulatum</em></span> samples obtained from different sources. A PCR-based random amplified polymorphic DNA (RAPD) assay was used to delineate polymorphisms among isolates in geographically diverse regions in Brazil. RAPD fingerprints revealed distinct DNA profiles and provided a high level of discrimination among <em>H. capsulatum</em> strains from different locations. Cluster I was composed of <em>H. capsulatum</em> isolates from the northeast region. The majority of strains from southeast and south were categorized as major cluster II. The strain 84564 from Rio de Janeiro State showed no genetic correlation to any of the isolates from the same state. The RAPD patterns of <em>H. capsulatum</em> isolates from Goias (Cluster III) were unrelated to DNA fingerprints observed among the other <em>H. capsulatum</em> strains (48% similarity). This study is the first report that stratifies the clusters of <em>H. capsulatum</em> strains from Brazil by molecular typing and associates them with the geographical origin.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.018","citationCount":"26","resultStr":"{\"title\":\"Genetic diversity of Histoplasma capsulatum strains in Brazil\",\"authors\":\"Rosely M. Zancopé-Oliveira, Patrícia Morais e Silva Tavares, Mauro de Medeiros Muniz\",\"doi\":\"10.1016/j.femsim.2005.05.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study establishes the genetic relatedness among Brazilian <span><em>Histoplasma capsulatum</em></span> samples obtained from different sources. A PCR-based random amplified polymorphic DNA (RAPD) assay was used to delineate polymorphisms among isolates in geographically diverse regions in Brazil. RAPD fingerprints revealed distinct DNA profiles and provided a high level of discrimination among <em>H. capsulatum</em> strains from different locations. Cluster I was composed of <em>H. capsulatum</em> isolates from the northeast region. The majority of strains from southeast and south were categorized as major cluster II. The strain 84564 from Rio de Janeiro State showed no genetic correlation to any of the isolates from the same state. The RAPD patterns of <em>H. capsulatum</em> isolates from Goias (Cluster III) were unrelated to DNA fingerprints observed among the other <em>H. capsulatum</em> strains (48% similarity). This study is the first report that stratifies the clusters of <em>H. capsulatum</em> strains from Brazil by molecular typing and associates them with the geographical origin.</p></div>\",\"PeriodicalId\":12220,\"journal\":{\"name\":\"FEMS immunology and medical microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.018\",\"citationCount\":\"26\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"FEMS immunology and medical microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0928824405001549\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405001549","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Genetic diversity of Histoplasma capsulatum strains in Brazil
This study establishes the genetic relatedness among Brazilian Histoplasma capsulatum samples obtained from different sources. A PCR-based random amplified polymorphic DNA (RAPD) assay was used to delineate polymorphisms among isolates in geographically diverse regions in Brazil. RAPD fingerprints revealed distinct DNA profiles and provided a high level of discrimination among H. capsulatum strains from different locations. Cluster I was composed of H. capsulatum isolates from the northeast region. The majority of strains from southeast and south were categorized as major cluster II. The strain 84564 from Rio de Janeiro State showed no genetic correlation to any of the isolates from the same state. The RAPD patterns of H. capsulatum isolates from Goias (Cluster III) were unrelated to DNA fingerprints observed among the other H. capsulatum strains (48% similarity). This study is the first report that stratifies the clusters of H. capsulatum strains from Brazil by molecular typing and associates them with the geographical origin.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
