SARS-CoV-2蛋白荧光构建体的综合文库及其在不同细胞类型中的初始特征

IF 2.4 4区生物学 Q4 CELL BIOLOGY Biology of the Cell Pub Date : 2021-03-05 DOI:10.1111/boc.202000158

Stéphanie Miserey-Lenkei, Katarina Trajkovic, Juan Martín D'Ambrosio, Amanda J Patel, Alenka Čopič, Pallavi Mathur, Kristine Schauer, Bruno Goud, Véronique Albanèse, Romain Gautier, Melody Subra, David Kovacs, Hélène Barelli, Bruno Antonny

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引用次数: 14

摘要

具有各种标签(如Strep、HA、Turbo)的SARS-CoV-2蛋白质粒的综合文库现已可用。它们能够识别SARS-CoV-2病毒与宿主蛋白质之间的许多潜在蛋白质-蛋白质相互作用。我们在此展示了一个与绿色和红色荧光蛋白融合的SARS CoV-2蛋白构建体的大文库，以及它们在各种人类细胞系(包括肺上皮细胞模型(A549, BEAS-2B))和出芽酵母中的初步表征。一些SARS-CoV-2蛋白的定位与它们与宿主蛋白的相互作用相匹配。其中包括Nsp13定位于中心体，Orf3a定位于核内体晚期，Orf9b定位于线粒体。结论和意义该文库有助于进一步的细胞研究，特别是通过成像技术。

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A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types

Background Information

Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein–protein interactions between the SARS-CoV-2 virus and host proteins.

Results

We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria.

Conclusions and Significance

This library should facilitate further cellular investigations, notably by imaging techniques.

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来源期刊

Biology of the Cell 生物-细胞生物学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal publishes original research articles and reviews on all aspects of cellular, molecular and structural biology, developmental biology, cell physiology and evolution. It will publish articles or reviews contributing to the understanding of the elementary biochemical and biophysical principles of live matter organization from the molecular, cellular and tissues scales and organisms. This includes contributions directed towards understanding biochemical and biophysical mechanisms, structure-function relationships with respect to basic cell and tissue functions, development, development/evolution relationship, morphogenesis, stem cell biology, cell biology of disease, plant cell biology, as well as contributions directed toward understanding integrated processes at the organelles, cell and tissue levels. Contributions using approaches such as high resolution imaging, live imaging, quantitative cell biology and integrated biology; as well as those using innovative genetic and epigenetic technologies, ex-vivo tissue engineering, cellular, tissue and integrated functional analysis, and quantitative biology and modeling to demonstrate original biological principles are encouraged.

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