{"title":"咖啡酸苯乙酯对乳腺癌细胞周期调控基因表达的影响。","authors":"Tuğçe Balc-Okcanoğlu, Sunde Yilma-Susluer, Cagla Kayabasi, Besra Ozme-Yelken, Cigir Biray-Avci, Cumhur Gunduz","doi":"10.22099/mbrc.2020.38811.1563","DOIUrl":null,"url":null,"abstract":"<p><p>We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to IC<sub>50</sub> dose and untreated control cells. Expressions of genes related to cell cycle control (<i>CCND2</i>, <i>RB1</i>, <i>ATM</i>, <i>CDC34</i>, <i>CDK5RAP1</i>) were evaluated by qRT-PCR by the LightCycler 480 System (Roche). <i>GAPDH</i> and <i>ACTB</i> housekeeping genes were used for the normalization of gene expressions. IC<sub>50</sub> value of CAPE in MCF-7 cells was calculated as 75µM. It was shown that IC<sub>50</sub> dose of CAPE induced significant upregulation in expressions of cell cycle control genes, compared to control cells. CAPE increases the expression of genes that are important in cell cycle control, suggesting that this component can be used as an effective chemopreventive agent in breast cancer cells.</p>","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936384/pdf/","citationCount":"6","resultStr":"{\"title\":\"The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells.\",\"authors\":\"Tuğçe Balc-Okcanoğlu, Sunde Yilma-Susluer, Cagla Kayabasi, Besra Ozme-Yelken, Cigir Biray-Avci, Cumhur Gunduz\",\"doi\":\"10.22099/mbrc.2020.38811.1563\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to IC<sub>50</sub> dose and untreated control cells. Expressions of genes related to cell cycle control (<i>CCND2</i>, <i>RB1</i>, <i>ATM</i>, <i>CDC34</i>, <i>CDK5RAP1</i>) were evaluated by qRT-PCR by the LightCycler 480 System (Roche). <i>GAPDH</i> and <i>ACTB</i> housekeeping genes were used for the normalization of gene expressions. IC<sub>50</sub> value of CAPE in MCF-7 cells was calculated as 75µM. It was shown that IC<sub>50</sub> dose of CAPE induced significant upregulation in expressions of cell cycle control genes, compared to control cells. CAPE increases the expression of genes that are important in cell cycle control, suggesting that this component can be used as an effective chemopreventive agent in breast cancer cells.</p>\",\"PeriodicalId\":19025,\"journal\":{\"name\":\"Molecular Biology Research Communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2021-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936384/pdf/\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Biology Research Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22099/mbrc.2020.38811.1563\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Biology Research Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22099/mbrc.2020.38811.1563","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells.
We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to IC50 dose and untreated control cells. Expressions of genes related to cell cycle control (CCND2, RB1, ATM, CDC34, CDK5RAP1) were evaluated by qRT-PCR by the LightCycler 480 System (Roche). GAPDH and ACTB housekeeping genes were used for the normalization of gene expressions. IC50 value of CAPE in MCF-7 cells was calculated as 75µM. It was shown that IC50 dose of CAPE induced significant upregulation in expressions of cell cycle control genes, compared to control cells. CAPE increases the expression of genes that are important in cell cycle control, suggesting that this component can be used as an effective chemopreventive agent in breast cancer cells.
期刊介绍：
“Molecular Biology Research Communications” (MBRC) is an international journal of Molecular Biology. It is published quarterly by Shiraz University (Iran). The MBRC is a fully peer-reviewed journal. The journal welcomes submission of Original articles, Short communications, Invited review articles, and Letters to the Editor which meets the general criteria of significance and scientific excellence in all fields of “Molecular Biology”.
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