{"title":"OFD1蛋白是选择性自噬中的一个新角色:这是纤毛/自噬之谜的另一个拼图。","authors":"Manuela Morleo, Brunella Franco","doi":"10.15698/cst2021.03.244","DOIUrl":null,"url":null,"abstract":"<p><p>The autophagy-lysosomal pathway is one of the main degradative routes which cells use to balance sources of energy. A number of proteins orchestrate the formation of autophagosomes, membranous organelles instrumental in autophagy. Selective autophagy, involving the recognition and removal of specific targets, is mediated by autophagy receptors, which recognize cargos and the autophagosomal membrane protein LC3 for lysosomal degradation. Recently, bidirectional crosstalk has emerged between autophagy and primary cilia, microtubule-based sensory organelles extending from cells and anchored by the basal body, derived from the mother centriole of the centrosome. The molecular mechanisms underlying the direct role of autophagic proteins in cilia biology and, conversely, the impact of this organelle in autophagy remains elusive. Recently, we uncovered the molecular mechanism by which the centrosomal/basal body protein OFD1 controls the LC3-mediated autophagic cascade. In particular, we demonstrated that OFD1 acts as a selective autophagy receptor by regulating the turnover of unc-51-like kinase (ULK1) complex, which plays a crucial role in the initiation steps of autophagosome biogenesis. Moreover, we showed that patients with a genetic condition caused by mutations in <i>OFD1</i> and associated with cilia dysfunction, display excessive autophagy and we demonstrated that autophagy inhibition significantly ameliorates the renal cystic phenotype in a conditional mouse model recapitulating the features of the disease (Morleo et al. 2020, EMBO J, doi: 10.15252/embj.2020105120). 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引用次数: 7
摘要
自噬-溶酶体途径是细胞用来平衡能量来源的主要降解途径之一。许多蛋白质协调自噬体的形成,自噬体是有助于自噬的膜细胞器。选择性自噬涉及特异性靶标的识别和去除,由自噬受体介导,自噬受体识别货物和自噬体膜蛋白LC3进行溶酶体降解。最近,自噬和初级纤毛之间出现了双向串扰,初级纤毛是基于微管的感觉细胞器,从细胞中延伸出来,由基底体锚定,源自中心体的母中心粒。自噬蛋白在纤毛生物学中的直接作用的分子机制,以及相反,这种细胞器在自噬中的影响仍然是难以捉摸的。最近,我们揭示了中心体/基底体蛋白OFD1控制lc3介导的自噬级联的分子机制。特别是,我们证明了OFD1通过调节unc-51样激酶(ULK1)复合物的周转,作为一种选择性自噬受体,在自噬体生物发生的起始步骤中起着至关重要的作用。此外,我们发现,由OFD1突变引起的与纤毛功能障碍相关的遗传病患者表现出过度的自噬,我们发现自噬抑制显著改善了重现该疾病特征的条件小鼠模型中的肾囊表型(Morleo et al. 2020, EMBO J, doi: 10.15252/embj.2020105120)。我们推测异常自噬可能是纤毛功能障碍引起的疾病的一些临床表现的基础。
The OFD1 protein is a novel player in selective autophagy: another tile to the cilia/autophagy puzzle.
The autophagy-lysosomal pathway is one of the main degradative routes which cells use to balance sources of energy. A number of proteins orchestrate the formation of autophagosomes, membranous organelles instrumental in autophagy. Selective autophagy, involving the recognition and removal of specific targets, is mediated by autophagy receptors, which recognize cargos and the autophagosomal membrane protein LC3 for lysosomal degradation. Recently, bidirectional crosstalk has emerged between autophagy and primary cilia, microtubule-based sensory organelles extending from cells and anchored by the basal body, derived from the mother centriole of the centrosome. The molecular mechanisms underlying the direct role of autophagic proteins in cilia biology and, conversely, the impact of this organelle in autophagy remains elusive. Recently, we uncovered the molecular mechanism by which the centrosomal/basal body protein OFD1 controls the LC3-mediated autophagic cascade. In particular, we demonstrated that OFD1 acts as a selective autophagy receptor by regulating the turnover of unc-51-like kinase (ULK1) complex, which plays a crucial role in the initiation steps of autophagosome biogenesis. Moreover, we showed that patients with a genetic condition caused by mutations in OFD1 and associated with cilia dysfunction, display excessive autophagy and we demonstrated that autophagy inhibition significantly ameliorates the renal cystic phenotype in a conditional mouse model recapitulating the features of the disease (Morleo et al. 2020, EMBO J, doi: 10.15252/embj.2020105120). We speculate that abnormal autophagy may underlie some of the clinical manifestations observed in the disorders ascribed to cilia dysfunction.
Cell StressBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
13.50
自引率
0.00%
发文量
21
审稿时长
15 weeks
期刊介绍:
Cell Stress is an open-access, peer-reviewed journal that is dedicated to publishing highly relevant research in the field of cellular pathology. The journal focuses on advancing our understanding of the molecular, mechanistic, phenotypic, and other critical aspects that underpin cellular dysfunction and disease. It specifically aims to foster cell biology research that is applicable to a range of significant human diseases, including neurodegenerative disorders, myopathies, mitochondriopathies, infectious diseases, cancer, and pathological aging.
The scope of Cell Stress is broad, welcoming submissions that represent a spectrum of research from fundamental to translational and clinical studies. The journal is a valuable resource for scientists, educators, and policymakers worldwide, as well as for any individual with an interest in cellular pathology. It serves as a platform for the dissemination of research findings that are instrumental in the investigation, classification, diagnosis, and therapeutic management of major diseases. By being open-access, Cell Stress ensures that its content is freely available to a global audience, thereby promoting international scientific collaboration and accelerating the exchange of knowledge within the research community.