橡果籽提取物银纳米颗粒膜制剂抗菌性能的设计与评价。

Q3 Medicine Polimery w medycynie Pub Date : 2020-07-01 DOI:10.17219/pim/130388
Michael A Odeniyi, Emmanuel Olusomoka, Olubusola A Odeniyi, Bukola C Adebayo-Tayo
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引用次数: 1

摘要

背景:植物提取物还原金属纳米颗粒提供了克服微生物耐药性的手段。将它们纳入适当的药物制剂将提高它们的便携性和易于管理。目的:以白螺旋藻种子甲醇提取物为盖层剂合成纳米银,并将其制成抗菌薄膜。材料与方法:采用DPPH(1,1-二苯基-2-苦味酰肼)法对白齿果(Blighia sapida K.D. Koenig)种子甲醇提取物进行植物化学筛选,并测定其抗氧化性能。以1 mM硝酸银水溶液(AgNO3)与甲醇提取物在烧瓶中反应,绿色合成了橡果籽提取物纳米银颗粒(ASAgNPs);在37°C下进行72 h的生物还原。所得到的纳米颗粒被冻干,并使用紫外可见分光光度法、傅里叶变换红外光谱法(FTIR)和显微摄影法进行表征。采用溶剂蒸发法制备淀粉和羧甲基纤维素纳米颗粒。采用琼脂孔扩散法对提取液、生物合成纳米颗粒和膜制剂进行了抗菌活性筛选。结果:猕猴桃甲醇籽提取物中含有皂苷、单宁、黄酮类化合物、萜类化合物和蒽醌类化合物。提取物具有显著的抗氧化性能。纳米颗粒和膜制剂比植物提取物具有更广泛的抑菌活性,对大肠杆菌ATCC 700728、伤寒沙门氏菌ATCC 14028、金黄色葡萄球菌ATCC 29213和铜绿假单胞菌ATCC 27853具有显著的抑菌活性。克鲁氏念珠菌、白色念珠菌和青霉也有活性。值得注意的是,青霉对氟康唑有抗性。结论:橡果籽提取物银纳米颗粒对多种病原菌具有协同抑菌作用。纳米颗粒的薄膜配方保留了这种抗菌活性,并允许产品以消费者准备好的形式呈现。
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Design and evaluation of the antimicrobial properties of ackee seed extract silver nanoparticle film formulations.

Background: Plant-extract-reduced metal nanoparticles provide means of overcoming microbial resistance. Incorporating them into appropriate pharmaceutical formulations will enhance their portability and ease of administration.

Objectives: To synthesize silver nanoparticles using methanol extracts of the seeds of Blighia sapida as capping agents and formulating the products in antimicrobial films.

Material and methods: Phytochemical screening of the methanol extract of Blighia sapida K.D. Koenig (ackee) seeds was performed and its antioxidant properties were determined using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. The green synthesis of ackee seed extract silver nanoparticles (ASAgNPs) was accomplished with reacting 1 mM of aqueous silver nitrate (AgNO3) and the methanol extract in a flask; the bioreduction was performed at 37°C for 72 h. The resulting nanoparticles were lyophilized and characterized using UV-visible spectrophotometry, Fourier-transform infrared spectroscopy (FTIR) and photomicrography. The nanoparticles were further formulated into films using starch and carboxymethyl cellulose using the solvent evaporation method. The extract, biosynthesized nanoparticles and film formulations were screened for antimicrobial activity against several pathogens using the agar well diffusion method.

Results: The methanol seed extracts of the ackee fruit contained saponins, tannins, flavonoids, terpenoids, and anthraquinones. The extract exhibited significant antioxidant properties. The nanoparticles and film formulations had a broader range of activity against microbes than the plant extract, exhibiting significant activity against Escherichia coli ATCC 700728, Salmonella typhi ATCC 14028, Staphylococcus aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853. Activity was also observed with Candida krusei, C. albicans, and Penicillium sp. It is noteworthy that this last organism showed resistance to fluconazole.

Conclusions: Ackee seed extract silver nanoparticles exhibited a synergistic antimicrobial activity against several pathogens. Film formulations of the nanoparticles retained this antimicrobial activity and allowed the product to be presented in a consumer-ready form.

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来源期刊
Polimery w medycynie
Polimery w medycynie Medicine-Medicine (all)
CiteScore
3.30
自引率
0.00%
发文量
9
审稿时长
53 weeks
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