用单粒子电子冷冻显微镜测定膜蛋白结构的两亲环境。

IF 7.2 2区 生物学 Q1 BIOPHYSICS Quarterly Reviews of Biophysics Pub Date : 2021-03-31 DOI:10.1017/S0033583521000044
Christel Le Bon, Baptiste Michon, Jean-Luc Popot, Manuela Zoonens
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引用次数: 12

摘要

在过去的十年中,由于单粒子电子冷冻显微镜(cryo-EM)具有划时代意义的技术进步以及样品制备的改进,膜蛋白(MPs)的结构生物学有了新的发展。目前的分析提供的范围和使用模式的各种类型的表面活性剂的低温电镜研究的概述。洋地黄苷、十二烷基麦芽糖苷、蛋白质基纳米圆盘、十二烷基麦芽糖苷-新戊二醇、糖薯蓣皂苷元和双酚类(APols)是玻璃化步骤中最常用的表面活性剂。表面活性剂的交换经常在聚乳酸提纯和栅格制备之间使用,每次研究新的聚乳酸都需要大量的优化。在过去的几年里,各种各样的表面活性剂和实验方法都证明了继续开发创新表面活性剂和优化样品制备条件的必要性。讨论了新型apol为EM应用提供的可能性。
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Amphipathic environments for determining the structure of membrane proteins by single-particle electron cryo-microscopy.

Over the past decade, the structural biology of membrane proteins (MPs) has taken a new turn thanks to epoch-making technical progress in single-particle electron cryo-microscopy (cryo-EM) as well as to improvements in sample preparation. The present analysis provides an overview of the extent and modes of usage of the various types of surfactants for cryo-EM studies. Digitonin, dodecylmaltoside, protein-based nanodiscs, lauryl maltoside-neopentyl glycol, glyco-diosgenin, and amphipols (APols) are the most popular surfactants at the vitrification step. Surfactant exchange is frequently used between MP purification and grid preparation, requiring extensive optimization each time the study of a new MP is undertaken. The variety of both the surfactants and experimental approaches used over the past few years bears witness to the need to continue developing innovative surfactants and optimizing conditions for sample preparation. The possibilities offered by novel APols for EM applications are discussed.

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来源期刊
Quarterly Reviews of Biophysics
Quarterly Reviews of Biophysics 生物-生物物理
CiteScore
12.90
自引率
1.60%
发文量
16
期刊介绍: Quarterly Reviews of Biophysics covers the field of experimental and computational biophysics. Experimental biophysics span across different physics-based measurements such as optical microscopy, super-resolution imaging, electron microscopy, X-ray and neutron diffraction, spectroscopy, calorimetry, thermodynamics and their integrated uses. Computational biophysics includes theory, simulations, bioinformatics and system analysis. These biophysical methodologies are used to discover the structure, function and physiology of biological systems in varying complexities from cells, organelles, membranes, protein-nucleic acid complexes, molecular machines to molecules. The majority of reviews published are invited from authors who have made significant contributions to the field, who give critical, readable and sometimes controversial accounts of recent progress and problems in their specialty. The journal has long-standing, worldwide reputation, demonstrated by its high ranking in the ISI Science Citation Index, as a forum for general and specialized communication between biophysicists working in different areas. Thematic issues are occasionally published.
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