Nazirah Bashir, Sheikh Bilal Ahmad, Muneeb U Rehman, Showkeen Muzamil, Rahil Razak Bhat, Manzoor Ur Rahman Mir, Gamal A Shazly, Mohamed A Ibrahim, Gehan M Elossaily, Abdelrahman Y Sherif, Mohsin Kazi
{"title":"姜酮(4-(4-羟基-3-甲基苯基)丁烷- 2 -1)通过调节炎症细胞因子和抗氧化剂调节佐剂诱导的类风湿性关节炎。","authors":"Nazirah Bashir, Sheikh Bilal Ahmad, Muneeb U Rehman, Showkeen Muzamil, Rahil Razak Bhat, Manzoor Ur Rahman Mir, Gamal A Shazly, Mohamed A Ibrahim, Gehan M Elossaily, Abdelrahman Y Sherif, Mohsin Kazi","doi":"10.1080/13510002.2021.1907518","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Ginger (<i>Zingiber officinale</i> Roscoe) is considered to be one of the most commonly consumed dietary condiments of the world. The present study was designed to explicate the protective role of zingerone; an active ingredient of ginger in complete Freund's adjuvant (FCA)-immunized arthritic rats.</p><p><strong>Methods: </strong>24 Wistar rats were divided into 4 groups with 6 rats each. Group I as control followed by group II, III and IV were treated with single intradermal injection of FCA (0.1 ml = 100 µg) to induce rheumatoid arthritis. Group III and IV were also administered with zingerone orally at 25 mg/kg b.w for 3 weeks at two different time points.</p><p><strong>Results: </strong>Adjuvant-treated rats exhibited a significant increase in lipid peroxidation and a reduction in the enzymatic antioxidants such as SOD, catalase and GPx, in the liver and joint tissues. Moreover, FCA inoculation resulted in the increase in levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and a decrease in IL-10 levels. Zingerone significantly reduced the levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and markedly increased IL-10 levels. Levels of antioxidant enzymes were also restored by zingerone treatment.</p><p><strong>Discussion: </strong>Oral administration of zingerone ameliorated inflammatory outburst and decreased oxidative stress, suggesting its role in the prevention of rheumatoid arthritis. Further mechanistic insights are necessary to study the exact mechanism involved.</p>","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":null,"pages":null},"PeriodicalIF":5.2000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13510002.2021.1907518","citationCount":"16","resultStr":"{\"title\":\"Zingerone (4-(four-hydroxy-3-methylphenyl) butane-two-1) modulates adjuvant-induced rheumatoid arthritis by regulating inflammatory cytokines and antioxidants.\",\"authors\":\"Nazirah Bashir, Sheikh Bilal Ahmad, Muneeb U Rehman, Showkeen Muzamil, Rahil Razak Bhat, Manzoor Ur Rahman Mir, Gamal A Shazly, Mohamed A Ibrahim, Gehan M Elossaily, Abdelrahman Y Sherif, Mohsin Kazi\",\"doi\":\"10.1080/13510002.2021.1907518\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Ginger (<i>Zingiber officinale</i> Roscoe) is considered to be one of the most commonly consumed dietary condiments of the world. The present study was designed to explicate the protective role of zingerone; an active ingredient of ginger in complete Freund's adjuvant (FCA)-immunized arthritic rats.</p><p><strong>Methods: </strong>24 Wistar rats were divided into 4 groups with 6 rats each. Group I as control followed by group II, III and IV were treated with single intradermal injection of FCA (0.1 ml = 100 µg) to induce rheumatoid arthritis. Group III and IV were also administered with zingerone orally at 25 mg/kg b.w for 3 weeks at two different time points.</p><p><strong>Results: </strong>Adjuvant-treated rats exhibited a significant increase in lipid peroxidation and a reduction in the enzymatic antioxidants such as SOD, catalase and GPx, in the liver and joint tissues. Moreover, FCA inoculation resulted in the increase in levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and a decrease in IL-10 levels. Zingerone significantly reduced the levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and markedly increased IL-10 levels. Levels of antioxidant enzymes were also restored by zingerone treatment.</p><p><strong>Discussion: </strong>Oral administration of zingerone ameliorated inflammatory outburst and decreased oxidative stress, suggesting its role in the prevention of rheumatoid arthritis. 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引用次数: 16
摘要
目的:生姜(Zingiber officinale Roscoe)被认为是世界上最常用的膳食调味品之一。本研究旨在阐明姜酮的保护作用;生姜在完全弗氏佐剂(FCA)免疫关节炎大鼠中的活性成分。方法:24只Wistar大鼠随机分为4组,每组6只。ⅰ组为对照,ⅱ、ⅲ、ⅳ组分别单次皮内注射FCA (0.1 ml = 100µg)诱导类风湿关节炎。第三组和第四组在两个不同的时间点口服生姜酮,剂量为25 mg/kg b.w,持续3周。结果:经佐剂处理的大鼠肝脏和关节组织中脂质过氧化作用明显增加,酶促抗氧化剂如SOD、过氧化氢酶和GPx含量明显降低。此外,接种FCA导致NF-κB、TGF-β、TNF-α、IL-1β、IL-6和Hs-CRP水平升高,IL-10水平降低。姜酮显著降低NF-κB、TGF-β、TNF-α、IL-1β、IL-6、Hs-CRP水平,显著升高IL-10水平。生姜酮处理也能恢复抗氧化酶水平。讨论:口服姜酮可改善炎症爆发,降低氧化应激,提示其在预防类风湿关节炎中的作用。要研究所涉及的确切机制,需要进一步的机制见解。
Zingerone (4-(four-hydroxy-3-methylphenyl) butane-two-1) modulates adjuvant-induced rheumatoid arthritis by regulating inflammatory cytokines and antioxidants.
Objective: Ginger (Zingiber officinale Roscoe) is considered to be one of the most commonly consumed dietary condiments of the world. The present study was designed to explicate the protective role of zingerone; an active ingredient of ginger in complete Freund's adjuvant (FCA)-immunized arthritic rats.
Methods: 24 Wistar rats were divided into 4 groups with 6 rats each. Group I as control followed by group II, III and IV were treated with single intradermal injection of FCA (0.1 ml = 100 µg) to induce rheumatoid arthritis. Group III and IV were also administered with zingerone orally at 25 mg/kg b.w for 3 weeks at two different time points.
Results: Adjuvant-treated rats exhibited a significant increase in lipid peroxidation and a reduction in the enzymatic antioxidants such as SOD, catalase and GPx, in the liver and joint tissues. Moreover, FCA inoculation resulted in the increase in levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and a decrease in IL-10 levels. Zingerone significantly reduced the levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and markedly increased IL-10 levels. Levels of antioxidant enzymes were also restored by zingerone treatment.
Discussion: Oral administration of zingerone ameliorated inflammatory outburst and decreased oxidative stress, suggesting its role in the prevention of rheumatoid arthritis. Further mechanistic insights are necessary to study the exact mechanism involved.
期刊介绍:
Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included.
While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.