{"title":"牙髓干细胞细胞外囊泡对大鼠颅骨骨缺损骨再生的影响。","authors":"Yuka Imanishi, Masaki Hata, Ryohei Matsukawa, Atsushi Aoyagi, Maiko Omi, Makoto Mizutani, Keiko Naruse, Shogo Ozawa, Masaki Honda, Tatsuaki Matsubara, Jun Takebe","doi":"10.1186/s41232-021-00163-w","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Extracellular vesicles (EVs) are known to be secreted by various cells. In particular, mesenchymal stem cell (MSC)-derived EVs (MSC-EVs) have tissue repair capacity and anti-inflammatory properties. Dental pulp stem cells (DPSCs), which are MSCs isolated from pulp tissue, are less invasive to the body than other MSCs and can be collected from young individuals. In this study, we investigated the efficacy of EVs secreted by DPSCs (DPSC-EVs) for bone formation.</p><p><strong>Methods: </strong>DPSC-EVs were isolated from the cell culture medium of DPSCs. DPSC-EVs were unilaterally injected along with collagen (COL), beta-tricalcium phosphate (β-TCP) or hydroxyapatite (HA) into rat calvarial bone defects. The effects of DPSC-EVs were analyzed by micro-computed tomography (micro-CT) and histological observation.</p><p><strong>Results: </strong>Micro-CT showed that administration of DPSC-EVs with the abovementioned scaffolds resulted in bone formation in the periphery of the defects. DPSC-EVs/COL specifically resulted in bone formation in the center of the defects. Histological observation revealed that DPSC-EVs/COL promoted new bone formation. 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DPSC-EVs/COL specifically resulted in bone formation in the center of the defects. Histological observation revealed that DPSC-EVs/COL promoted new bone formation. 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引用次数: 2
摘要
背景:细胞外囊泡(EVs)是由多种细胞分泌的。特别是间充质干细胞(MSC)衍生的EVs (MSC-EVs)具有组织修复能力和抗炎特性。牙髓干细胞(DPSCs)是一种从牙髓组织中分离出来的间充质干细胞，与其他间充质干细胞相比，对身体的侵袭性较小，可以从年轻人身上采集。在这项研究中，我们研究了由dpsc分泌的EVs (DPSC-EVs)对骨形成的影响。方法:从dpsc细胞培养基中分离dpsc - ev。将DPSC-EVs与胶原(COL)、β-磷酸三钙(β-TCP)或羟基磷灰石(HA)一起单侧注入大鼠颅骨骨缺损。通过显微计算机断层扫描(micro-CT)和组织学观察分析dpsc - ev的作用。结果:Micro-CT显示，将dpsc - ev与上述支架一起使用可使缺损周围骨形成。dpsc - ev /COL特异性地导致缺损中心的骨形成。组织学观察显示DPSC-EVs/COL促进新骨形成。DPSCs - ev /COL对骨缺损部位的影响与DPSCs/COL移植几乎相同。结论:这些结果提示dpsc - ev可能是骨组织再生的有效工具。
Efficacy of extracellular vesicles from dental pulp stem cells for bone regeneration in rat calvarial bone defects.
Background: Extracellular vesicles (EVs) are known to be secreted by various cells. In particular, mesenchymal stem cell (MSC)-derived EVs (MSC-EVs) have tissue repair capacity and anti-inflammatory properties. Dental pulp stem cells (DPSCs), which are MSCs isolated from pulp tissue, are less invasive to the body than other MSCs and can be collected from young individuals. In this study, we investigated the efficacy of EVs secreted by DPSCs (DPSC-EVs) for bone formation.
Methods: DPSC-EVs were isolated from the cell culture medium of DPSCs. DPSC-EVs were unilaterally injected along with collagen (COL), beta-tricalcium phosphate (β-TCP) or hydroxyapatite (HA) into rat calvarial bone defects. The effects of DPSC-EVs were analyzed by micro-computed tomography (micro-CT) and histological observation.
Results: Micro-CT showed that administration of DPSC-EVs with the abovementioned scaffolds resulted in bone formation in the periphery of the defects. DPSC-EVs/COL specifically resulted in bone formation in the center of the defects. Histological observation revealed that DPSC-EVs/COL promoted new bone formation. Administration of DPSC-EVs/COL had almost the same effect on the bone defect site as transplantation of DPSCs/COL.
Conclusions: These results suggest that DPSC-EVs may be effective tools for bone tissue regeneration.
期刊介绍：
Inflammation and Regeneration is the official journal of the Japanese Society of Inflammation and Regeneration (JSIR). This journal provides an open access forum which covers a wide range of scientific topics in the basic and clinical researches on inflammation and regenerative medicine. It also covers investigations of infectious diseases, including COVID-19 and other emerging infectious diseases, which involve the inflammatory responses.
Inflammation and Regeneration publishes papers in the following categories: research article, note, rapid communication, case report, review and clinical drug evaluation.
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