[通过人胎盘和血清中dna基因表达谱鉴定子痫前期-一项初步研究]。

A Ahr, U Holtrich, T Karn, A Reitter, A Rody, M Kaufmann, R Gätje
{"title":"[通过人胎盘和血清中dna基因表达谱鉴定子痫前期-一项初步研究]。","authors":"A Ahr,&nbsp;U Holtrich,&nbsp;T Karn,&nbsp;A Reitter,&nbsp;A Rody,&nbsp;M Kaufmann,&nbsp;R Gätje","doi":"10.1055/s-2006-933377","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Preeclampsia is associated with significant maternal and fetal morbidity and mortality. The etiology remains unclear. For the accurate diagnosis and the prevention of preeclampsia it seems to be important to find a diagnostic tool that identifies risk patients before symptoms occur. With a new approach, the cDNA-Array analysis, human placentas and blood from preeclamptic and healthy pregnant women were examined for differentially expressed genes to find typical genes expression profiles.</p><p><strong>Material and methods: </strong>In this pilot study, cDNA array analysis with a 19 200 gene array of placenta and blood samples from three preeclamptic patients have been performed to classify this samples based on expression patterns.</p><p><strong>Results: </strong>Comparing normal placenta and blood from healthy delivered women (n = 4), a subset of 200 genes repeatedly found to be differentially expressed in preeclampsia. The placenta and blood samples from preeclampsia were accurately grouped by their individual gene expression patterns.</p><p><strong>Conclusions: </strong>These results suggest that the use of cDNA array is a tool to identify gene expression patterns in preeclampsia. With this set of differentially expressed genes in conjunction with sample clustering algorithms the identification of preeclampsia in placenta or blood samples is possible.</p>","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"128 3","pages":"138-42"},"PeriodicalIF":0.0000,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-2006-933377","citationCount":"2","resultStr":"{\"title\":\"[Identification of preeclampsia by cDNA-gene expression profiling in human placentas and serum -- a pilot study].\",\"authors\":\"A Ahr,&nbsp;U Holtrich,&nbsp;T Karn,&nbsp;A Reitter,&nbsp;A Rody,&nbsp;M Kaufmann,&nbsp;R Gätje\",\"doi\":\"10.1055/s-2006-933377\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Preeclampsia is associated with significant maternal and fetal morbidity and mortality. The etiology remains unclear. For the accurate diagnosis and the prevention of preeclampsia it seems to be important to find a diagnostic tool that identifies risk patients before symptoms occur. With a new approach, the cDNA-Array analysis, human placentas and blood from preeclamptic and healthy pregnant women were examined for differentially expressed genes to find typical genes expression profiles.</p><p><strong>Material and methods: </strong>In this pilot study, cDNA array analysis with a 19 200 gene array of placenta and blood samples from three preeclamptic patients have been performed to classify this samples based on expression patterns.</p><p><strong>Results: </strong>Comparing normal placenta and blood from healthy delivered women (n = 4), a subset of 200 genes repeatedly found to be differentially expressed in preeclampsia. The placenta and blood samples from preeclampsia were accurately grouped by their individual gene expression patterns.</p><p><strong>Conclusions: </strong>These results suggest that the use of cDNA array is a tool to identify gene expression patterns in preeclampsia. With this set of differentially expressed genes in conjunction with sample clustering algorithms the identification of preeclampsia in placenta or blood samples is possible.</p>\",\"PeriodicalId\":23881,\"journal\":{\"name\":\"Zentralblatt fur Gynakologie\",\"volume\":\"128 3\",\"pages\":\"138-42\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1055/s-2006-933377\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt fur Gynakologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1055/s-2006-933377\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Gynakologie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1055/s-2006-933377","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

摘要

目的:先兆子痫与显著的母婴发病率和死亡率相关。病因尚不清楚。为了准确诊断和预防先兆子痫,在症状出现之前找到一种诊断工具来识别风险患者似乎很重要。采用一种新的方法,dna - array分析,检测人类胎盘和来自子痫前期和健康孕妇的血液中的差异表达基因,以找到典型的基因表达谱。材料与方法:对3例子痫前期患者的胎盘和血液样本进行19200个基因序列的cDNA阵列分析,根据表达模式对这些样本进行分类。结果:比较正常胎盘和健康分娩妇女的血液(n = 4),反复发现200个基因子集在子痫前期存在差异表达。胎盘和子痫前期的血液样本根据其个体基因表达模式准确分组。结论:这些结果表明,cDNA阵列是一种鉴定子痫前期基因表达模式的工具。这组差异表达基因与样本聚类算法相结合,可以在胎盘或血液样本中识别子痫前期。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
[Identification of preeclampsia by cDNA-gene expression profiling in human placentas and serum -- a pilot study].

Objective: Preeclampsia is associated with significant maternal and fetal morbidity and mortality. The etiology remains unclear. For the accurate diagnosis and the prevention of preeclampsia it seems to be important to find a diagnostic tool that identifies risk patients before symptoms occur. With a new approach, the cDNA-Array analysis, human placentas and blood from preeclamptic and healthy pregnant women were examined for differentially expressed genes to find typical genes expression profiles.

Material and methods: In this pilot study, cDNA array analysis with a 19 200 gene array of placenta and blood samples from three preeclamptic patients have been performed to classify this samples based on expression patterns.

Results: Comparing normal placenta and blood from healthy delivered women (n = 4), a subset of 200 genes repeatedly found to be differentially expressed in preeclampsia. The placenta and blood samples from preeclampsia were accurately grouped by their individual gene expression patterns.

Conclusions: These results suggest that the use of cDNA array is a tool to identify gene expression patterns in preeclampsia. With this set of differentially expressed genes in conjunction with sample clustering algorithms the identification of preeclampsia in placenta or blood samples is possible.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
[Units of measurement]. [Not significant--what now?]. [Fluorescence diagnosis and photodynamic therapy with 5-aminolevulinic acid induced protoporphyrin IX in gynecology: an overview]. [Chemotherapy for metastatic breast cancer]. [The expert patient: medical consequences].
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1