Establishment of nuclear transfer embryonic stem cell lines from adult somatic cells by nuclear transfer and its application.

Nuclear transfer can be used to generate embryonic stem cell (ntESC) lines from a patient's own somatic cells. We have shown that ntESCs can be generated relatively easily from a variety of mouse genotypes and cell types of both sexes, even though it may be more difficult to generate clones directly. Several reports have already demonstrated that ntESCs can be used in regenerative medicine in order to rescue immunodeficient or infertile phenotypes. However, it is unclear whether ntES cells are identical to fertilized embryonic stem cells (ESCs). This review seeks to describe the phenotype and possible abnormalities of ntESC lines.