[水螅培养水对微囊性铜绿菌的抑制作用及其机制]。

植物生理与分子生物学学报 Pub Date : 2006-12-01
Li-Xin Wang, Ling Zhang, Yu-Xia Zhang, Chun-Yan Jin, Chang-Mei Lu, Guo-Rong Wu
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引用次数: 0

摘要

本文研究了弯孢杆菌培养水对铜绿单胞菌生长的抑制作用及其机理。在处理过程中,绿僵菌的光合速率下降,呼吸速率和SOD活性随处理的进行先升高后降低。其膜透性也显著增加。透射电镜显示,铜绿单胞菌的细胞膜、类囊体薄片和髓样核的超微结构被严重破坏。只有用乙醚提取提取物才能观察到抑制作用。醚提取物越多,抑菌效果越好。提示轮状芽胞杆菌对铜绿假单胞菌的抑制作用是通过向水中排泄物质来实现的。GC/MS分析结果表明,该醚提取物主要由1,2-苯二甲酸二异辛酯、邻苯二甲酸二丁酯和1,2-苯二甲酸丁基2-甲基丙酯组成。
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[The inhibitory effect of Hydrilla verticillata culture water on Microcystic aeruginosa and its mechanism].

This paper studied on the effect and mechanism that the growth of M. aerugonsa was markedly inhibited by the H. verticillata culture water. During treatment, the photosynthetic rate of M. aerugonsa declined, while its respiratory rate and SOD activity increased firstly, then decreased as the treatment went on. Its membrane permeability also increased significantly. TEM photographs showed that the ultrastructure of cell membrane, thylakoid lamella and pith nucleoid of M. aerugonsa were destroyed severely. Inhibitory effects could be observed only when the extracts were extracted by ether. The more extracts from ether, the better inhibitory effect observed. It suggested that the inhibitory effects of H. verticillata on M. aeruginosa were through excreting substances into water. GC/MS analytic result showed that the ether extract mainly consisted of 1,2-benzenedicarboxylic acid diisooctyl ester, dibutyl phthalate, and 1,2-benzenedicarboxylic acid butyl 2-methylpropyl ester.

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