{"title":"[大鼠前庭内器官马赫受体ml-m5亚基的表达]。","authors":"Huanmao Cheng, Weijia Kong, Qi Yao","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression of mACh receptor ml - m5 subunits in the vestibular endorgans of rat.</p><p><strong>Method: </strong>Gene specific primers used were based upon the rat mACh receptor ml-m5 subunits sequence. Reverse transcription polymerase chain reaction (RT-PCR) was carried out on the vestibular endorgans. The PCR products were directly sequenced.</p><p><strong>Result: </strong>It was shown that mACh receptor ml - m5 subunits mRNA was detected and sequenced from vestibular endorgans by RT-PCR technique. Sequence analysis of the PCR products confirmed identity to rat mACh receptor ml--m5 subunits cDNA sequence in the predicted region.</p><p><strong>Conclusion: </strong>Present data confirmed the expression of ACh receptor ml-m5 subunits in the vestibular endorgans of rat, giving the direct evidence to date that ACh is one of the important neurotransmitters in the peripheral vestibular system of rat.</p>","PeriodicalId":79680,"journal":{"name":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","volume":"20 22","pages":"1027-9"},"PeriodicalIF":0.0000,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Expression of mACh receptor ml-m5 subunits in the vestibular endorgans of rat].\",\"authors\":\"Huanmao Cheng, Weijia Kong, Qi Yao\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the expression of mACh receptor ml - m5 subunits in the vestibular endorgans of rat.</p><p><strong>Method: </strong>Gene specific primers used were based upon the rat mACh receptor ml-m5 subunits sequence. Reverse transcription polymerase chain reaction (RT-PCR) was carried out on the vestibular endorgans. The PCR products were directly sequenced.</p><p><strong>Result: </strong>It was shown that mACh receptor ml - m5 subunits mRNA was detected and sequenced from vestibular endorgans by RT-PCR technique. Sequence analysis of the PCR products confirmed identity to rat mACh receptor ml--m5 subunits cDNA sequence in the predicted region.</p><p><strong>Conclusion: </strong>Present data confirmed the expression of ACh receptor ml-m5 subunits in the vestibular endorgans of rat, giving the direct evidence to date that ACh is one of the important neurotransmitters in the peripheral vestibular system of rat.</p>\",\"PeriodicalId\":79680,\"journal\":{\"name\":\"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology\",\"volume\":\"20 22\",\"pages\":\"1027-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Expression of mACh receptor ml-m5 subunits in the vestibular endorgans of rat].
Objective: To investigate the expression of mACh receptor ml - m5 subunits in the vestibular endorgans of rat.
Method: Gene specific primers used were based upon the rat mACh receptor ml-m5 subunits sequence. Reverse transcription polymerase chain reaction (RT-PCR) was carried out on the vestibular endorgans. The PCR products were directly sequenced.
Result: It was shown that mACh receptor ml - m5 subunits mRNA was detected and sequenced from vestibular endorgans by RT-PCR technique. Sequence analysis of the PCR products confirmed identity to rat mACh receptor ml--m5 subunits cDNA sequence in the predicted region.
Conclusion: Present data confirmed the expression of ACh receptor ml-m5 subunits in the vestibular endorgans of rat, giving the direct evidence to date that ACh is one of the important neurotransmitters in the peripheral vestibular system of rat.
分享
京公网安备 11010802042870号
京ICP备2023020795号-1
求助内容：
应助结果提醒方式：
扫码关注我们
