{"title":"植物硫化碱-α对烟草'亮黄 2 号'悬浮细胞增殖的刺激作用","authors":"Jun-Ying Chen, Yan-Hui Yang, Hai-Xia Xu, Xi-Yong Cheng, Xin-Jian Chen","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>'Bright Yellow 2' ('BY-2') tobacco (Nicotiana tabacum L.) suspension cells could not proliferate even with proper 2, 4-D concentration (0.6 mg/L) in the medium, when the initial cell density is low. However, the cells could divide and grow normally if conditioned medium (CM) was added to the medium, and the rate of proliferation of cells was proportional to the quantities of CM supplied. The same results were obtained, when the CM was replaced by synthesized phytosulfokine-alpha (PSK-alpha), a sulfated pentapeptide, PSK-alpha was found in CM of 'BY-2' cells by MS identification. From the significant linear relationship between rate of cell proliferation (measured by OD600 value) and concentrations (0.05 nmol/L-10 micromol/L) of PSK-alpha, it can be seen that the 'BY-2' suspended cells are the ideal plant material for bioassay of PSK-alpha. This result suggests that the PSK-alpha might be involved in promoting the proliferation of 'BY-2' suspension cells.</p>","PeriodicalId":64030,"journal":{"name":"植物生理与分子生物学学报","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2007-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Stimulatory effect of phytosulfokine-alpha on the proliferation of tobacco 'Bright Yellow 2' suspension cells.\",\"authors\":\"Jun-Ying Chen, Yan-Hui Yang, Hai-Xia Xu, Xi-Yong Cheng, Xin-Jian Chen\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>'Bright Yellow 2' ('BY-2') tobacco (Nicotiana tabacum L.) suspension cells could not proliferate even with proper 2, 4-D concentration (0.6 mg/L) in the medium, when the initial cell density is low. However, the cells could divide and grow normally if conditioned medium (CM) was added to the medium, and the rate of proliferation of cells was proportional to the quantities of CM supplied. The same results were obtained, when the CM was replaced by synthesized phytosulfokine-alpha (PSK-alpha), a sulfated pentapeptide, PSK-alpha was found in CM of 'BY-2' cells by MS identification. From the significant linear relationship between rate of cell proliferation (measured by OD600 value) and concentrations (0.05 nmol/L-10 micromol/L) of PSK-alpha, it can be seen that the 'BY-2' suspended cells are the ideal plant material for bioassay of PSK-alpha. This result suggests that the PSK-alpha might be involved in promoting the proliferation of 'BY-2' suspension cells.</p>\",\"PeriodicalId\":64030,\"journal\":{\"name\":\"植物生理与分子生物学学报\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"植物生理与分子生物学学报\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"植物生理与分子生物学学报","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
烟草（Nicotiana tabacum L.）悬浮细胞'亮黄 2 号'（'BY-2'）在初始细胞密度较低时，即使培养基中含有适当浓度（0.6 mg/L）的 2，4-D，细胞也无法增殖。但是，如果在培养基中加入条件培养基（CM），细胞就能正常分裂和生长，而且细胞的增殖速度与提供的 CM 数量成正比。通过 MS 鉴定，在'BY-2'细胞的 CM 中发现了 PSK-alpha。从细胞增殖率（用 OD600 值测量）与 PSK-alpha 浓度（0.05 nmol/L-10 micromol/L）之间明显的线性关系可以看出，'BY-2'悬浮细胞是进行 PSK-alpha 生物测定的理想植物材料。这一结果表明，PSK-α可能参与了促进'BY-2'悬浮细胞增殖的过程。
Stimulatory effect of phytosulfokine-alpha on the proliferation of tobacco 'Bright Yellow 2' suspension cells.
'Bright Yellow 2' ('BY-2') tobacco (Nicotiana tabacum L.) suspension cells could not proliferate even with proper 2, 4-D concentration (0.6 mg/L) in the medium, when the initial cell density is low. However, the cells could divide and grow normally if conditioned medium (CM) was added to the medium, and the rate of proliferation of cells was proportional to the quantities of CM supplied. The same results were obtained, when the CM was replaced by synthesized phytosulfokine-alpha (PSK-alpha), a sulfated pentapeptide, PSK-alpha was found in CM of 'BY-2' cells by MS identification. From the significant linear relationship between rate of cell proliferation (measured by OD600 value) and concentrations (0.05 nmol/L-10 micromol/L) of PSK-alpha, it can be seen that the 'BY-2' suspended cells are the ideal plant material for bioassay of PSK-alpha. This result suggests that the PSK-alpha might be involved in promoting the proliferation of 'BY-2' suspension cells.
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