{"title":"马己糖激酶II基因cDNA及近端启动子序列分析。","authors":"Tetsuo Sato, Takuya Itou, Go Sato, Yuki Kobayashi, Hideki Endo, Takeo Sakai","doi":"10.1080/10425170601136648","DOIUrl":null,"url":null,"abstract":"<p><p>In order to investigate the utilization of glucose in equine skeletal muscle, we determined the coding and proximal promoter sequences of the hexokinase type II (HKII) gene in thoroughbred horse, Grevy's zebra and Hartmann's mountain zebra. The deduced amino acid sequence of thoroughbred horse HKII showed 100, 100, 94.4, 92.7 and 92.6% identities with Grevy's zebra, Hartmann's mountain zebra, human, mouse and rat HKIIs, respectively. In equine HKIIs, specific amino acid substitutions, Ile 159 and Arg 610, were found in the potential binding site for glucose. In addition, the nucleotide sequence of the equine proximal promoter has a number of conserved putative binding sites for transcription factor.</p>","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170601136648","citationCount":"1","resultStr":"{\"title\":\"Sequencing of cDNA and proximal promoter of equine hexokinase II gene.\",\"authors\":\"Tetsuo Sato, Takuya Itou, Go Sato, Yuki Kobayashi, Hideki Endo, Takeo Sakai\",\"doi\":\"10.1080/10425170601136648\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In order to investigate the utilization of glucose in equine skeletal muscle, we determined the coding and proximal promoter sequences of the hexokinase type II (HKII) gene in thoroughbred horse, Grevy's zebra and Hartmann's mountain zebra. The deduced amino acid sequence of thoroughbred horse HKII showed 100, 100, 94.4, 92.7 and 92.6% identities with Grevy's zebra, Hartmann's mountain zebra, human, mouse and rat HKIIs, respectively. In equine HKIIs, specific amino acid substitutions, Ile 159 and Arg 610, were found in the potential binding site for glucose. In addition, the nucleotide sequence of the equine proximal promoter has a number of conserved putative binding sites for transcription factor.</p>\",\"PeriodicalId\":11197,\"journal\":{\"name\":\"DNA sequence : the journal of DNA sequencing and mapping\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/10425170601136648\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"DNA sequence : the journal of DNA sequencing and mapping\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10425170601136648\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA sequence : the journal of DNA sequencing and mapping","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10425170601136648","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Sequencing of cDNA and proximal promoter of equine hexokinase II gene.
In order to investigate the utilization of glucose in equine skeletal muscle, we determined the coding and proximal promoter sequences of the hexokinase type II (HKII) gene in thoroughbred horse, Grevy's zebra and Hartmann's mountain zebra. The deduced amino acid sequence of thoroughbred horse HKII showed 100, 100, 94.4, 92.7 and 92.6% identities with Grevy's zebra, Hartmann's mountain zebra, human, mouse and rat HKIIs, respectively. In equine HKIIs, specific amino acid substitutions, Ile 159 and Arg 610, were found in the potential binding site for glucose. In addition, the nucleotide sequence of the equine proximal promoter has a number of conserved putative binding sites for transcription factor.
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