评估坦桑尼亚维多利亚湖地区卫生设施艾滋病毒检测的质量保证。

F M Mashauri, J E Siza, M M Temu, J T Mngara, C Kishamawe, J M Changalucha
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引用次数: 25

摘要

坦桑尼亚目前正在实施抗逆转录病毒治疗方案,目标是到2008年使大约40万名符合条件的艾滋病毒感染者接受治疗。这将涉及对大量人群进行筛查,这将需要非实验室人员参与进行艾滋病毒检测。为了保证可靠和高质量的艾滋病毒检测结果,需要确保从标本采集、检测和报告结果都遵循质量保证程序。鉴于上述情况,进行了一项调查,以评估坦桑尼亚维多利亚湖地区卫生设施中艾滋病毒检测的质量保证。总共调查了89个保健设施(29家医院、34个保健中心、9个药房和17个自愿和咨询检测中心)。只有三家(10.3%)卫生机构报告使用统一II型酶联免疫吸附试验进行艾滋病毒诊断。据报告,所有其他卫生设施都在使用Capillus和decide艾滋病毒快速检测试剂盒。5个(5.6%)卫生设施实验室进行了CD4计数。21家(63.6%)医院实施了内部质量控制。据报告,54个(60.7%)设施可随时获得艾滋病毒检测试剂盒。只有16个(18%)卫生设施制定了标准操作程序。13家(14.6%)卫生设施报告了设备校准系统。所有保健设施都提供咨询服务,所有咨询人员都接受了为期6周的强制性培训课程。这些调查结果表明,维多利亚湖地区的大多数卫生机构在艾滋病毒检测中没有遵守质量保证程序。因此,有必要对进行艾滋病毒检测的实验室建立一个监测系统,以确保质量保证程序的完成。进行艾滋病毒检测的人员应定期接受再培训,以应对新技术,并确保遵循质量保证程序。
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Assessment of quality assurance in HIV testing in health facilities in Lake Victoria zone, Tanzania.

Tanzania is currently implementing the antiretroviral treatment programme, and has a target of putting about 400,000 eligible HIV infected individuals on treatment by 2008. This will involve screening a large number of people, which will require non-laboratory personnel to be involved in doing HIV testing. In order to guarantee reliable and quality HIV test results, there is a need to ensure that quality assurance (QA) procedures are followed from specimen collection, testing and reporting of results. In light of the above a survey was conducted to assess QA in HIV testing in health facilities in Lake Victoria zone, Tanzania. A total of 89 health facilities (29 hospitals, 34 health centres, 9 dispensaries and 17 voluntary and counselling testing centres) were surveyed. Only three (10.3%) health facilities reported performing Uniform II ELISA for HIV diagnosis. All other health facilities reported to be using HIV rapid tests Capillus and Determine. Five (5.6%) of health facility laboratories performed CD4 counts. Internal quality control (IQC) were performed in 21 (63.6%) of the hospitals. Kits for HIV testing were reported to be readily available by 54 (60.7%) of the facilities. Only 16 (18%) of the health facilities had standard operating procedures in place. Systems of equipment calibration were reported by 13 (14.6%) of the health facilities. Counselling services were available in all health facilities and all counsellors had received the 6-week mandatory training course. These findings show that most of health facilities in the Lake Victoria zone do not adhere to QA procedures in HIV testing. There is therefore, a need to establish a monitoring system to laboratories performing HIV testing for the purpose of ensuring QA procedures are done. Personnel doing HIV testing should be re-trained at a regular basis to cope with new techniques and ensure QA procedures are followed.

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