[NaCl胁迫对转基因杨树外源基因表达的影响]。

植物生理与分子生物学学报 Pub Date : 2007-10-01
Hui-Jing Han, Fa-Yong Zhang, Min-Sheng Yang, Hai-Yong Liang, Hua-Xin Zhang
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引用次数: 0

摘要

以高转抗虫基因‘杨树741’和转抗虫基因741(抗虫基因和Ri质粒T-DNA)组培植株为材料,研究了BtCry1Ac抗虫基因和根生基因的表达及其对NaCl胁迫的响应。结果表明,IAA和GA含量迅速增加,植物根数和根长度增加后减少生根和激素合成相关基因在国际扶轮本文分别插入到基因组的杨树(Figs.2 3 8和9)。株高、根数、叶绿素含量、IAA和GA含量逐渐下降与氯化钠应力强度(Figs.1 2 4 - 6,转罗尔基因植株各项指标变化幅度小于转Bt基因植株和非转Bt基因植株。NaCl胁迫下转rol基因植株的Bt毒素蛋白含量显著升高(图7)。结果表明,外源基因的表达随环境条件的变化而变化。
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[Effects of NaCl stress on the exogenous gene expression in transgenic poplar plants].

The expressions of BtCry1Ac insect-resistance genes and rhizogenesis genes and their response to NaCl stress were studied using tissue culture plants of high transgenic insect-resistant 'poplar 741' and transpolygenes 741 (insect-resistant genes and T-DNA of Ri plasmid). The results showed that IAA and GA contents increased quickly, plant root number increased and root length reduced after rhizogenesis and hormone synthesis related gene in Ri T-DNA were inserted into the genome of poplar (Figs.2, 3, 8 and 9). Plant height, root number, chlorophyll content, IAA and GA contents decreased gradually with an increase in NaCl stress intensity (Figs.1, 2, 4-6, 8 and 9). Apiece index change extent of transgenic rol gene plant was smaller than transgenic Bt gene plant and non-transgenic plant. Bt toxin protein content of transgenic rol gene plant increased significantly under NaCl stress (Fig.7). Our results indicate that the expressions of the foreign genes changed with the changes of the environmental conditions.

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