酿酒酵母对Zn2+的生物吸附特性

Can Chen, Jian-Long Wang
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引用次数: 0

摘要

目的:研究酿酒酵母完整细胞对Zn2+的生物吸附特性及吸附过程中阳离子的释放。方法:采用间歇吸附法测定吸附平衡及等温线。采用原子吸附分光光度计(AAS)测定Zn2+浓度。结果:当Zn2+初始浓度为0.08 ~ 0.8 mmol/L时,初始pH为自然pH(约5.65),吸附剂浓度约为1 g/L, Zn2+的生物吸附量为74.8 ~ 654.8 micromol/g。pH值升高0.55 ~ 1.28,细胞内离子(K+、Mg2+、Na+、Ca2+)在Zn2+生物吸附过程中释放。结论:离子交换是吸附Zn2+的机制之一。酿酒酵母的生物量是去除水溶液中Zn2+的潜在生物吸附剂。在付诸实际应用之前,还有很多工作要做。
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Characteristics of Zn2+ biosorption by Saccharomyces cerevisiae.

Objective: To investigate the characteristics of Zn2+ biosorption and the release of cations during the process of Zn2+ biosorption by intact cells of Saccharomyces cerevisiae.

Methods: The batch adsorption test was used to study the biosorption equilibrium and isotherm. Zn2+ concentration was measured with atomic adsorption spectrophotometer (AAS) AAS 6 Vario.

Results: When the initial concentration of Zn2+ ranged between 0.08 and 0.8 mmol/L, the initial pH was natural (about 5.65), the sorbent concentration was about 1 g/L and the capacity of Zn2+ biosorption was from 74.8 to 654.8 micromol/g. The pH value increased by 0.55-1.28 and the intracellular cations (K+, Mg2+, Na+, Ca2+) of the cells were released during the process of Zn2+ biosorption.

Conclusion: Ion exchange was one of the mechanisms for Zn2+ biosorption. The biomass of Saccharomyces cerevisiae is a potential biosorbent for the removal of Zn2+ from aqueous solution. More work needs to be done before putting it into practical application.

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