用于高效基因传递的聚乙烯亚胺衍生纳米颗粒。

A Pathak, S Patnaik, K C Gupta
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引用次数: 12

摘要

在非病毒介导的基因治疗中,安全有效地将治疗基因导入生物体细胞已成为一项具有挑战性的任务。在这里,支化聚乙烯亚胺(bPEI, 25 kDa)通过与阴离子多糖(如海藻酸,Al和透明质酸,HA)的静电相互作用转化为纳米粒子。通过改变阴离子多糖的量合成了一个小的PEI-Al和PEI-HA纳米颗粒文库,并对它们的大小、表面电荷、细胞毒性、转染效率等进行了评价。与天然PEI和标准转染试剂相比,这两种纳米颗粒均表现出更高的细胞活力和转染效率。研究了PEI-HA纳米颗粒在肿瘤诱导小鼠体内的靶向作用(4.6%)。
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Polyethylenimine derived nanoparticles for efficient gene delivery.

Introduction of therapeutic genes into the cells of an organism in a safe and efficient way has become a challenging task in non-viral mediated gene therapy. Here, branched polyethylenimine (bPEI, 25 kDa) was converted into nanoparticles through electrostatic interactions with anionic polysaccharides (e.g. alginic acid, Al and hyaluronic acid, HA). A small library of PEI-Al and PEI-HA nanoparticles was synthesized by varying the amounts of anionic polysaccharides and evaluated in terms of their size, surface charge, cytotoxicity, transfection efficiency, etc. Both the series of nanoparticles exhibited higher cell viability and transfection efficiency as compared to native PEI and the standard transfection reagents. In vivo targeting efficacy of PEI-HA(4.6%) nanoparticles was examined in tumor induced mice.

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