HAC1 mRNA的核磁共振研究。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp135

Ikumi Kawahara, Kaichiro Haruta, Chojiro Kojima, Yoshiyuki Tanaka

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摘要

HAC1是酵母中与未折叠蛋白反应(UPR)信号通路相关的转录因子。内质网(Endoplasmic reticulum, ER)上HAC1 mRNA的加工在UPR信号通路中起着关键作用，但加工酶Ire1p对HAC1 mRNA的识别机制尚不清楚。本文采用核磁共振(NMR)技术研究HAC1 mRNA的溶液结构，重点研究HAC1 mRNA中Ire1p识别位点的结构。从NOESY谱中，我们找到了HAC1 mRNA 5'加工区的亚质子信号，发现该区域形成茎环结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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NMR studies of HAC1 mRNA.

HAC1 is a transcription factor related to Unfolded Protein Response (UPR) signaling in yeast. Processing of HAC1 mRNA on Endoplasmic reticulum (ER) plays a key role in UPR signaling pathway, but the recognition mechanism of HAC1 mRNA by processing enzyme Ire1p is still unclear. Here, the solution structure of HAC1 mRNA was investigated by Nuclear Magnetic Resonance (NMR) spectroscopy, focusing on the structure of the recognition site of Ire1p in HAC1 mRNA. From the NOESY spectrum, imino proton signals of 5' processing regions of HAC1 mRNA were assigned and it was found that this region forms the stem-loop structure.

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Nucleic acids symposium series (2004)

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