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引用次数: 1
摘要
我们已经开发出在5'和/或3'端腺嘌呤磷酸化环己烷的2'- o -甲基rna。这些2'- o -甲基RNA与较长的靶RNA形成的双工比与较短的靶RNA形成的双工更不稳定。我们试图通过合成末端鸟嘌呤修饰的2'- o -甲基rna来提高短rna的选择性杂交性能。
Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization.
We have developed 2'-O-methyl-RNAs having phosphorylated cyclohexane at the 5' and/or 3'-terminal adenine. These 2'-O-methyl-RNAs formed less stable duplex with the longer target RNA than that with the short target RNA. We tried to improve the short-RNA selective hybridization property by the synthesis of 2'-O-methyl-RNAs having terminal guanine modifications.
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