非天然咪唑吡啶嘧啶:萘啶碱基对:DNA聚合酶的选择性掺入和延伸反应。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp063

Noriaki Minakawa, Shintaro Ogata, Mayumi Takahashi, Akira Matsuda

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引用次数: 1

摘要

我们在这里描述了(i)酶对咪唑吡啶(Im):萘啶(Na)碱基对的识别结果，以及(ii) DNA聚合酶在Im:Na碱基对之后进一步的引物延伸反应。在检测的碱基对中，ImN(O):NaO(N)碱基对被Klenow片段exo(-) [F(exo(-))]选择性识别为互补碱基对。然而，该DNA聚合酶在ImN(O):NaO(N)碱基对后不催化引物延伸反应。因此，我们对DNA聚合酶进行筛选，以促进引物延伸反应，提高碱基对识别的选择性。因此，B家族DNA聚合酶，特别是深喷口(exo(-))，似乎最有希望实现这一目的。

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Unnatural imidazopyridopyrimidine:naphthyridine base pairs: selective incorporation and extension reaction by DNA polymerases.

We describe herein the results of (i) enzymatic recognition for imidazopyridopyrimidine (Im):naphthyridine (Na) base pairs and (ii) further primer extension reactions after the Im:Na base pairs by DNA polymerases. Among the base pairs examined, ImN(O):NaO(N) base pair was rather selectively recognized by Klenow fragment exo(-) [F(exo(-))]as complementary base. However, this DNA polymerase did not catalyze primer extension reactions after the ImN(O):NaO(N) base pair. Therefore, we carried out a screening of DNA polymerases to promote the primer extension reaction as well as to improve the selectivity of base pair recognition. As a result, a family B DNA polymerase, especially Deep Vent (exo(-)), seemed most promising for this purpose.

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Nucleic acids symposium series (2004)

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