Leonhard Geist, Paola Martinelli, Shereena Mohideen-Ali, Patrick Werni, Gerhard Fischer, Julian E. Fuchs, Klaus Rumpel, Moriz Mayer
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NMR applications to find and progress TREX1 binders
Inhibition of the exonuclease activity of Three-prime Repair EXonuclease 1 (TREX1) has emerged as a potential novel immunotherapeutic strategy. Herein we describe our screening approach to find initial low-affinity fragment hits for TREX1 from our 19F library and the use of both ligand- and target-based NMR methods for hit confirmation and validation. In a further step to support the early hit expansion stage we describe our setup of a 19F competition assay for KI determination of analogs.
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