Claire Larroux, Bryony Fahey, Maja Adamska, Gemma S Richards, Marie Gauthier, Kathryn Green, Erica Lovas, Bernard M Degnan
{"title":"两栖动物全载原位杂交。","authors":"Claire Larroux, Bryony Fahey, Maja Adamska, Gemma S Richards, Marie Gauthier, Kathryn Green, Erica Lovas, Bernard M Degnan","doi":"10.1101/pdb.prot5096","DOIUrl":null,"url":null,"abstract":"<p><p>INTRODUCTIONDevelopmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.</p>","PeriodicalId":10835,"journal":{"name":"CSH protocols","volume":" ","pages":"pdb.prot5096"},"PeriodicalIF":0.0000,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/pdb.prot5096","citationCount":"21","resultStr":"{\"title\":\"Whole-mount in situ hybridization in amphimedon.\",\"authors\":\"Claire Larroux, Bryony Fahey, Maja Adamska, Gemma S Richards, Marie Gauthier, Kathryn Green, Erica Lovas, Bernard M Degnan\",\"doi\":\"10.1101/pdb.prot5096\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>INTRODUCTIONDevelopmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.</p>\",\"PeriodicalId\":10835,\"journal\":{\"name\":\"CSH protocols\",\"volume\":\" \",\"pages\":\"pdb.prot5096\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1101/pdb.prot5096\",\"citationCount\":\"21\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"CSH protocols\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1101/pdb.prot5096\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"CSH protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/pdb.prot5096","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
INTRODUCTIONDevelopmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
