食品真实性分析的三种替代工具的适用性:转基因生物鉴定。

A Burrell, C Foy, M Burns
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引用次数: 21

摘要

确保食品上正确标注了来自转基因生物(gmo)的成分是制造商、零售商和执法机构面临的一个问题。DNA检测食品真实性的方法通常使用聚合酶链反应(PCR), PCR产物可以用毛细管或凝胶电泳检测。本研究检验了三种实验室电泳仪器(Agilent Bioanalyzer 2100, Lab901 TapeStation和Shimadzu MCE-202 multi)基于先前验证的方案使用PCR检测转基因生物的适用性。虽然观察到偏差和精度的性能特征存在微小差异,但所有三种仪器都证明了它们在使用该方案筛选转基因成分方面的适用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Applicability of three alternative instruments for food authenticity analysis: GMO identification.

Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients.

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