用于猕猴神经科学研究的无框架立体定向MRI技术。

Q4 Medicine Open Neuroimaging Journal Pub Date : 2011-01-01 Epub Date: 2011-11-18 DOI:10.2174/1874440001105010198
David J Dubowitz, Miriam Scadeng
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引用次数: 8

摘要

MRI已广泛应用于非人类灵长类动物研究的预先计划神经科学程序。然而,定向成像研究在立体定位空间主要依赖于使用立体定位框架或与神经成像共同注册基准标记。在这项研究中,我们提出了一种简单的方法,其中MRI数据集与定义法兰克福立体定向基线平面的骨地标对齐,而不需要立体定向框架或额外的外部基准。为了方便定位MRI扫描上的骨标志(眶下缘,外骨听道),我们讨论了额外的成像标志(眼中平面,颞下颌关节),这些标志提供了补充和容易看到的参考点。采用0.7mm各向同性分辨率的三维快速梯度MRI图像,对8只恒河猴无框架MRI立体定向技术进行了评价。1)比较传统立体定向框架与无框架MRI技术中基准标记物立体定向坐标的差异(n=2)。2)比较无框架MRI技术与动物在MRI兼容的立体定位框架中获得的MRI脑区立体定位坐标的差异(n=4)。3)无框架MRI技术进一步完善,以相对于电极微驱动器的立体定向坐标在硬脑膜记录腔内规定电极穿透。将MRI坐标的差异与电极微驱动进行比较(n=3)。在无框架MRI技术和传统立体定位框架之间,基准标记的平均定位差异为1.6 +/- 0.6 mm。在无框架技术和mri兼容的立体定位框架之间,大脑解剖定位差异为2.8 +/- 2.2 mm,主要误差来源是矢状面俯仰旋转。当旋转被移除时,这种定位差异减小到0.5 +/- 0.6 mm。硬脑膜记录腔内电极束的无框MRI坐标在电极微驱动读数0.5mm +/- 0.2 mm范围内。这种简单的技术提供了在单个动物中精确计划手术和神经生理记录的能力,并使用公开可用的软件定义大脑解剖结构和电极或注射束的位置,而不需要专用的mri兼容定位硬件。减少了对深度麻醉的需求(传统立体定位框架的必要条件)使得该技术更适合功能性MRI研究。由于每种动物都提供骨骼标记来定义它们自己的立体定位空间,因此这种技术很容易适用于其他物种。
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A frameless stereotaxic MRI technique for macaque neuroscience studies.

MRI has achieved widespread use for preplanning neuroscience procedures for non-human primate studies. However, orienting imaging studies in stereotaxic space has relied primarily on using a stereotaxic frame or co-registering fiducial markers with the neuroimaging. In this study, we present a simple approach in which the MRI dataset is aligned to the bony landmarks that define the Frankfurt stereotaxic baseline plane, without the need for a stereotaxic frame or additional external fiducials. To facilitate localizing the bony landmarks (infraorbital margin, external bony auditory meatus) on the MRI scans additional imaging landmarks (mid ocular plane, temporomandibular joint) are discussed that provide supplementary and readily visible points of reference. The frameless MRI stereotaxic technique was evaluated in 8 rhesus macaque monkeys using 3D fast gradient echo MRI images with 0.7mm isotropic resolution. 1) Difference in stereotaxic coordinates of fiducial markers was compared between a traditional stereotaxic frame and the frameless MRI technique (n=2). 2) Differences in stereotaxic coordinates for cerebral regions were compared between the frameless MRI technique and MRI obtained with the animal positioned in a MRI-compatible stereotaxic frame (n=4). 3) The frameless MRI technique was further refined to prescribe electrode penetrations within a dural recording chamber in stereotaxic coordinates relative to the electrode microdrive. Differences in MRI coordinates were compared with the electrode microdrive (n=3). Mean localization of fiducial markers differed by 1.6 +/- 0.6 mm between the frameless MRI technique and a traditional stereotaxic frame. Between the frameless technique and an MRI-compatible stereotaxic frame, localization of cerebral anatomy differed by 2.8 +/- 2.2 mm with the primary source of error being a pitch-up rotation in the sagittal plane. This localization difference was reduced to 0.5 +/- 0.6 mm when this rotation was removed. Frameless MRI coordinates for electrode tracts within the dural recording chamber were within 0.5mm +/- 0.2 mm of the electrode microdrive readings. This simple technique provides the ability to accurately plan surgery and neurophysiological recordings in an individual animal, and to define the location of cerebral anatomy and electrode or injection tracts using publically available software, and without the need for dedicated MRI-compatible localization hardware. The reduced need for deep anesthesia (a necessity with traditional stereotaxic frames) makes the technique more amenable for functional MRI studies. Since each animal provides the bony landmarks to define their own stereotaxic space, this technique is readily applicable to other species.

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来源期刊
Open Neuroimaging Journal
Open Neuroimaging Journal Medicine-Radiology, Nuclear Medicine and Imaging
CiteScore
0.70
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0.00%
发文量
3
期刊介绍: The Open Neuroimaging Journal is an Open Access online journal, which publishes research articles, reviews/mini-reviews, and letters in all important areas of brain function, structure and organization including neuroimaging, neuroradiology, analysis methods, functional MRI acquisition and physics, brain mapping, macroscopic level of brain organization, computational modeling and analysis, structure-function and brain-behavior relationships, anatomy and physiology, psychiatric diseases and disorders of the nervous system, use of imaging to the understanding of brain pathology and brain abnormalities, cognition and aging, social neuroscience, sensorimotor processing, communication and learning.
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