鲍曼不动杆菌对羟基苯乙酸 3-羟化酶还原酶成分的结晶和初步 X 射线分析。

Worrapoj Oonanant, Jeerus Sucharitakul, Pimchai Chaiyen, Jirundon Yuvaniyama
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引用次数: 0

摘要

鲍曼不动杆菌(Acinetobacter baumannii)的对羟基苯乙酸 3-羟化酶(HPAH)催化对羟基苯乙酸(HPA)在正位发生羟化反应,生成 3,4-二羟基苯乙酸(DHPA)。鲍曼不动杆菌的 HPAH 是一种双组分黄素蛋白,由一个较小的还原酶(C(1)组分)和一个较大的加氧酶(C(2)组分)组成。C(1) 组份以游离形式向 C(2) 组份提供还原黄素,供其羟化。此外,HPA 还能与 C(1) 结合,在不发生羟基化的情况下提高黄素还原率。重组的 C(1) 成分被纯化,并在 295 K 温度下用微批方法结晶。该晶体属于正交空间群 P2(1)2(1)2(1),单位晶胞参数 a = 47.78、b = 59.92、c = 211.85 Å,每个不对称单元含有两个分子的 C(1)。
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Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii.

p-Hydroxyphenylacetate 3-hydroxylase (HPAH) from Acinetobacter baumannii catalyzes the hydroxylation of p-hydroxyphenylacetate (HPA) at the ortho position to yield 3,4-dihydroxyphenylacetate (DHPA). HPAH from A. baumannii is a two-component flavoprotein consisting of a smaller reductase (C(1)) component and a larger oxygenase (C(2)) component. The C(1) component supplies a reduced flavin in its free form to the C(2) counterpart for hydroxylation. In addition, HPA can bind to C(1) and enhance the flavin-reduction rate without becoming hydroxylated. The recombinant C(1) component was purified and crystallized using the microbatch method at 295 K. X-ray diffraction data were collected to 2.3 Å resolution using synchrotron radiation on the BL13B1 beamline at NSRRC, Taiwan. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 47.78, b = 59.92, c = 211.85 Å, and contained two molecules of C(1) per asymmetric unit.

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期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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